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Effect of Blood Sampling Method During a Mating Time in Male Camels (Dromedary Camels)


Affiliations
1 Department of Medical Biotechnology, Faculty of Biotechnology, University of Al‑Qadisiyah,, Iraq
2 Department of Surgery and Obstetrics, Faculty of Veterinary Medicine, University of Al‑Qadisiyah, Iraq

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This study was to shed light on levels of testosterone and cortisol hormones in male dromedary camels during the mating time, by collection the blood samples using the manual method and compared with the remote‑controlled blood sampling (RBS) method, to assess whether either these method had affected changes in the concentration of the hormones or not. The blood samples were collected from fifteen adult male camels, via two experiment with one‑day intervals: first experiment by manual method during three periods (pre‑mating, mating, and post‑mating) one hour between each period, and the second experiment by the RBS in the same protocol. The serum testosterone and cortisol concentrations of all animals were determined via ELISA technique. The result which appearance a significant difference in the mating time used RBS compared with manual method. These findings might be due to the withdrawal of blood remotely which could cause a reduction of excitement in animals using the manual blood sampling at the presence of veterinarians, so it was considered as an ideal method to measure hormonal concentrations, especially in experiments which need accurate results.


Keywords

Camels, Cortisol, Mating, Remote‑Controlled Blood Sampling, Testosterone.
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Abstract Views: 327




  • Effect of Blood Sampling Method During a Mating Time in Male Camels (Dromedary Camels)

Abstract Views: 327  | 

Authors

Alaakamil Abdulla
Department of Medical Biotechnology, Faculty of Biotechnology, University of Al‑Qadisiyah,, Iraq
Ali Habeeb Jaber AL‑bdeery
Department of Surgery and Obstetrics, Faculty of Veterinary Medicine, University of Al‑Qadisiyah, Iraq
Basim Hameed Abed Ali
Department of Surgery and Obstetrics, Faculty of Veterinary Medicine, University of Al‑Qadisiyah, Iraq

Abstract


This study was to shed light on levels of testosterone and cortisol hormones in male dromedary camels during the mating time, by collection the blood samples using the manual method and compared with the remote‑controlled blood sampling (RBS) method, to assess whether either these method had affected changes in the concentration of the hormones or not. The blood samples were collected from fifteen adult male camels, via two experiment with one‑day intervals: first experiment by manual method during three periods (pre‑mating, mating, and post‑mating) one hour between each period, and the second experiment by the RBS in the same protocol. The serum testosterone and cortisol concentrations of all animals were determined via ELISA technique. The result which appearance a significant difference in the mating time used RBS compared with manual method. These findings might be due to the withdrawal of blood remotely which could cause a reduction of excitement in animals using the manual blood sampling at the presence of veterinarians, so it was considered as an ideal method to measure hormonal concentrations, especially in experiments which need accurate results.


Keywords


Camels, Cortisol, Mating, Remote‑Controlled Blood Sampling, Testosterone.



DOI: https://doi.org/10.37506/v11%2Fi1%2F2020%2Fijphrd%2F193958