Ethanol Extract with Black Cumin (Nigella Sativa) Against sFlt‑1 Level and VEGF Serum on Laboratory Mice with Preeclampsia

Introduction: Preeclampsia is one of the complications that occur in pregnancies. This study was aimed to study the factors that affect the of giving ethanol extract with black cumin (Nigella sativa) against sFlt‑1 level and VEGF serum on laboratory mice induced preeclampsia. Method: Laboratory experimental research with post test only control group design. This study used 30 BALB/C laboratory mice, divided into 6 groups, namely negative controls: pregnant mice injected serum from normal pregnant women, positive controls; mice modeled preeclampsia, and treatment groups 1, 2, 3 and 4 are preeclampsia mice received a dose of 500 mg, 1000 mg, 1500 mg and 2000 mg/kg weight of Nigella sativa ethanol extract for 5 days. Statistical analysis using ANOVA Result: The mean serum sFlt‑1 level in mice modeled preeclampsia and treatment group dose 500mg, 1000mg, 1500mg and 2000mg (2510.3+182.2 pg/mL, 2142.5+171.9 pg/mL, 1309+161.3 p/mL, and 1500+169.9, respectively) pg/mL) showed a significant difference (p<0.05) and found a decrease in serum sFlt‑1 levels with increasing doses. The mean serum VEGF levels in preeclampsia mice and treatment groups were 500 mg, 1000 mg, 1500 mg and 2000 mg (50.25±2.85b pg/mL, 60.18±4.81c pg/mL respectively, 71.89±2.38d pg/mL, 66.51±1.87 e pg/mL) showed a significant difference (p<0.05) and found an increase in serum VEGF levels as the dose increased. Conclusion: Giving of Black Cumin extract (Nigella sativa) decreases serum sFlt‑1 levels and increases serum VEGF levels in preeclampsia mice model and the effect is dependent dose.