Indian Journal of Public Health Research & Development

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Microwave tissue Processing


Affiliations
1 Post Graduate Student, Department of Oral Pathology and Microbiology Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, India
2 Professor, Department of Oral Pathology and Microbiology, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, India
3 Reader, Department of Oral Pathology and Microbiology, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, India
4 Professor and Head of the Department, Department of Oral Pathology and Microbiology, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, India
     

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Conventional tissue fixation and processing is as old as 100 years and still remains the gold standard in which all new technologies and methods are need to be assessed. Tissue processing is one of the important steps for obtaining good thin sections without artifacts. Though conventional tissue-processing methods are most commonly followed, they are well-known as very laborious and tedious procedures. Microwaves is a form of electromagnetic wave-induced heat, when applied in histotechnology, reproducibly yields histolologic material of similar or superior quality than conventional processing methods, making it popular in the recent years. Microwave irradiation of tissue during fixation and subsequent histochemical staining procedures significantly reduces the time required for incubation in fixation and staining solutions.

Keywords

Tissue Processing, Microwave, Staining.
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  • Microwave tissue Processing

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Authors

F. Ryhanath Gulshan
Post Graduate Student, Department of Oral Pathology and Microbiology Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, India
N. Aravindha Babu
Professor, Department of Oral Pathology and Microbiology, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, India
Jayasri Krupaa
Reader, Department of Oral Pathology and Microbiology, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, India
K. M. K. Masthan
Professor and Head of the Department, Department of Oral Pathology and Microbiology, Sree Balaji Dental College and Hospital, Bharath Institute of Higher Education and Research, India

Abstract


Conventional tissue fixation and processing is as old as 100 years and still remains the gold standard in which all new technologies and methods are need to be assessed. Tissue processing is one of the important steps for obtaining good thin sections without artifacts. Though conventional tissue-processing methods are most commonly followed, they are well-known as very laborious and tedious procedures. Microwaves is a form of electromagnetic wave-induced heat, when applied in histotechnology, reproducibly yields histolologic material of similar or superior quality than conventional processing methods, making it popular in the recent years. Microwave irradiation of tissue during fixation and subsequent histochemical staining procedures significantly reduces the time required for incubation in fixation and staining solutions.

Keywords


Tissue Processing, Microwave, Staining.