Correlation between Bio-Film Formation and Carbapenem Resistant Enterobacteriaceae Isolates Obtained from Various Clinical Specimens

Introduction: The ability to form bio-film is a universal trait of bacteria by attaching to the surfaces. Carbapenem resistance in Enterobacteriaceae might be endorsed by bio-film formation which augmented colonization of pathogens. Aim: To correlate between bio-film formation and carbapenem resistant Enterobacteriaceae isolates obtained from various clinical specimens and to compare the qualitative and quantitative assay for bio-film production. Method: Study was conducted on 150 strains of Enterobacteriaceae isolates. of these, carbapenem resistant Enterobacteriaceae isolates were subjected for bio-film formation by Modified Congo red agar method, tube adherence method and Tissue culture plate method. Results: Carbapenem resistant Enterobacteriaceae strains were found to be 60.66% and the rate of biofilm producers was 75.53% by any of the phenotypic method. Tissue culture plate method was found to be (67.6%) better than Modified Congo Red agar method (54.9%) and Tube adherence method (39.4%). The highest number of bio-film producers was isolated from urinary tract infections (36.61%). Conclusion: TCP method is most reliable, precise and sensitive method for detection of bio-film formation by Enterobacteriaceae isolates and is ideal to use as a general screening tool to detect bio-film production.