Antibacterial Activity of Sappan Wood (Caesalpinia Sappan L.) against Aggregatibacter Actinomycetemcomitans and Porphyromonas Gingivalis

The aim of this study was to find the Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of sappan wood ethanol extract (Caesalpinia sappan L.) toward the growth of A. actinomycetemcomitans and P. gingivalis. The randomized posttest only control group design was applied to this study. The sappan wood ethanol extract was conducted with maceration method using 96% ethanol. Diluted to 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, 0.78%, and 0.39% concentration. The MIC and MBC values of sappan wood ethanol extract toward A. actinomycetemcomitans and P. gingivalis was then known via the evaluation for Colony Forming Units (CFUs) in MH medium. The MIC and MBC of sappan wood ethanol extract toward A. actinomycetemcomitans and P. gingivalis was at 1.56% and 3.125% concentration. The ethanol extract of sappan wood reduces the number of bacterial colonies significantly at p=0.00. This study concluded that the growth of A. actinomycetemcomitans and P. gingivalis can be inhibited by sappan wood ethanol extract (Caesalpinia sappan L.) at MIC 1.56% and MBC 3.125% concentration.