Analysis of the Expression Toll-Like Receptor 4 (TLR4) in Chronic Suppurative Otitis Media with and without Cholesteatoma

In recent years, the increasing incidence of chronic suppurative otitis media (CSOM) diseases worldwide is often associated with the role of TLR4 as a component of innate immunity. This study aimed to analyze the TLR4 levels in patients with chronic suppurative otitis media with and without cholesteatoma. This study was conducted using the cross-sectional technique on 30 patients divided into two groups. The first group is CSOM with cholesteatoma that consisted of 10 patients and the second group isCSOM without cholesteatoma that consisted of 20 patients. Each sample was examined for TLR4 levels and bacterial identification cultures in secretions and several samples were also examined for TLR4 levels in the middle ear mucosa. TLR4 levels were examined by the ELISA method.

The results showed that TLR4 levels in middle ear secretions and mucosa of CSOM patients with cholesteatoma were higher than chronic suppurative otitis media without cholesteatoma but there were no significant differences. TLR4 levels in mucosal samples are higher than secret samples but there are no significant differences.There were no significant differences in TLR4 levels in both secret and mucosal samples between groups of CSOM with cholesteatoma and CSOM without cholesteatoma. There were no significant differencesin TLR4 levels betweenthe secret and mucosal sample in both CSOM with and without cholesteatoma.