Identification of Some Dermatophytes Isolated by PCR Technique in Misan Province/Iraq

Dermatophytes are a complex group of fungi, which include three genera Trichophyton, Epidermophyton and Microsporum. These fungi have the ability to degrade the keratin layer resulting in dermatophytosis, also known as Tinea or Ringworm. Because conventional method used to diagnose dermatophytes are slow and unspecialized, there is a need to use improved diagnostic method. Specialized primers were used OPAA11, OPD18, OPAA17 and OPU15 and the DNA sequence of the nuclear ribosome regions ITS1 and ITS4 were used. DNA was extracted for 14 isolates from dermatophytes (were isolated from patients in Misan province/southern Iraq Misan province/southern Iraq)and amplified by PCR for identify at species level by sequencing from the ribosomes using the MEGA program of mapping of the studied fungi to know the evolutionary relationships where the results of the genetic tree showed the existence of complex relationships between dermatophytes. The aim of this study was to identify different types of skin fungi as quickly and accurately as possible.