Indian Journal of Public Health Research & Development

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Molecular Detection of Human Cytomegalovirus and Immunohistochemical Expression of BCL2 in Lymph Nodes from Hodgkin’s Lymphoma


Affiliations
1 Assist Prof. Dr., College of Medicine, University of Baghdad, Iraq
2 Prof. Dr., College of Medicine, University of Baghdad, Iraq
3 Lecturer Dr., University of Al-Hilla Islamic, Iraq
4 Prof. Dr. College of Science, University of Babylon, Iraq
     

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Background: A lifelong persistence of latent human cytomegalovirus (hCMV) infection may result and/or associated with Hodgkin’s lymphoma (HL). The B-cell lymphoma-2 (BCL2) is an anti-apoptotic molecule expressed in non-neoplastic lymphoid tissue (hyperplastic lymph node) while down-regulated in normal germinal center B cells. Objective: Is to analyze the concordant impact of BCL2 expression and hCMV infection on tissues from a group of patients with Hodgkin’s lymphoma.

Patients and Method: Eighty formalin-fixed, paraffin-embedded lymph node tissue-biopsies enrolled in this study; 40 from HLand 40 with unremarkable pathological changes. Detection of hCMV was done by chromogenic in situ hybridization (CISH) technique while immunohistochemistry (IHC) was used for the BCL2 protein expression.

Results: In HL, the hCMV-positiveCISH and the BCL2-positive IHC reactions were detected in 35% and 42.5% of tissues, respectively. The correlation between hCMV and BCL2 was highly significant (P = 0.002).

Conclusions: The significant co-expression of BCL2 and hCMV genes in HL tissues could point for their possible role in either lymph node pathogenesis or carcinogenesis.


Keywords

hCMV, Hodgkin’s Lymphoma, BCL2, CISH, IHC.
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  • Molecular Detection of Human Cytomegalovirus and Immunohistochemical Expression of BCL2 in Lymph Nodes from Hodgkin’s Lymphoma

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Authors

Haitham Ahmed Al-Rubaie
Assist Prof. Dr., College of Medicine, University of Baghdad, Iraq
Saad Hasan Mohammed Ali
Prof. Dr., College of Medicine, University of Baghdad, Iraq
Mustafa Jaffar Hussein
Lecturer Dr., University of Al-Hilla Islamic, Iraq
Shakir H. Mohammed Al-Alwany
Prof. Dr. College of Science, University of Babylon, Iraq

Abstract


Background: A lifelong persistence of latent human cytomegalovirus (hCMV) infection may result and/or associated with Hodgkin’s lymphoma (HL). The B-cell lymphoma-2 (BCL2) is an anti-apoptotic molecule expressed in non-neoplastic lymphoid tissue (hyperplastic lymph node) while down-regulated in normal germinal center B cells. Objective: Is to analyze the concordant impact of BCL2 expression and hCMV infection on tissues from a group of patients with Hodgkin’s lymphoma.

Patients and Method: Eighty formalin-fixed, paraffin-embedded lymph node tissue-biopsies enrolled in this study; 40 from HLand 40 with unremarkable pathological changes. Detection of hCMV was done by chromogenic in situ hybridization (CISH) technique while immunohistochemistry (IHC) was used for the BCL2 protein expression.

Results: In HL, the hCMV-positiveCISH and the BCL2-positive IHC reactions were detected in 35% and 42.5% of tissues, respectively. The correlation between hCMV and BCL2 was highly significant (P = 0.002).

Conclusions: The significant co-expression of BCL2 and hCMV genes in HL tissues could point for their possible role in either lymph node pathogenesis or carcinogenesis.


Keywords


hCMV, Hodgkin’s Lymphoma, BCL2, CISH, IHC.



DOI: https://doi.org/10.37506/v11%2Fi2%2F2020%2Fijphrd%2F195193