A Real-Time Polymerase Chain Reactionassay for the Simultaneous Detection of Sexually Transmitted Pathogens in Women

Genital tract infection is a major source of morbidity in sexually active individuals. Reported rates of STD underestimate the exact problem of infection because the majority of STDs are asymptomatic and therefore go undetected especially in our community. The aim of this study was to investigate for the prevalence STD pathogens in females. 184 urogenital swabscollected from outpatient femalesthen analyzed by Real-Time PCR for eight different pathogens (Neisseria gonorrhoeae, Chlamydia trachomatis, Herpes simplex virus, Trichomonas vaginalis, Hepatitis viruses B & C, Mycoplasma genitalium and Human papillomavirus).90.21% of samples resulted positive for one of the targets named above. PCR is a beneficial diagnostic technique for STDs since it has a highly specific and sensitive. the most prevalence pathogen was Human papilloma virus 40.96%.