Indian Journal of Public Health Research & Development

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In Vitro Effects of Cadmium Chloride on Steroid Profiles of Post-vitellogenic Ovary in the Catfish Heteropneustes Fossilis


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1 Research Scholar, Zoology Section, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi
     

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Cadmium (Cd) is a known endocrine disruptor with the ability to affect the production of hormones involved in the regulation of reproductive processes. The purpose of this study was to evaluate invitro effects of cadmium chloride (CdCl2) exposure on steroid levels in post-vitellogenic follicles of catfish ovary. Pieces of ovarian tissues (500mg) were incubated in culture medium in the presence or absence (control) of CdCl2 (0,0.01,0.1,1,3 and 10μg/ml) for 12 or 24hr. Estradiol-17β (E2) and testosterone were measured by ELISA. Other steroids like progestins (progesterone -P4; 17-OH-progesterone-17-P and 17, 20 β-dihydroxyprogesterone-17, 20 β-DP) and corticosteroids (cortisol, 21-deoxycortisol, corticosterone and deoxycorticosterone) were quantified by High Performance Liquid Chromatography. The results show that Cd produced biphasic effects on E2, T, P4, 17,20β-DP, cortisol, deoxycorticosterone, and 21-deoxycortisol, stimulatory at lower concentrations and inhibitory at higher concentrations. In contrast, 17-P and corticosterone were inhibited in a dose-dependent manner. Thus, Cd can influence ovarian steroidogenesis adversely, affecting gametogenesis and ovulation resulting in the decline of fish population.

Keywords

Cadmium, Ovary, Invitro Steroid Levels, Endocrine Disruptors, Catfish
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  • Ebrahim M. Ultrastructural changes investigation on sperm exposed to cadmium, copper and mercury by scanning electron microscope. J. Shaheed Sadoughi Univ. Med. Sci. Health Serv. 2004 11(4): 70-78.

  • Patra RC, Swarup D, Sharma MC, Naresh R. Trace mineral profile in blood and hair from cattle environmentally exposed to lead and cadmium around different industrial units. Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine. 2006 53: 511-517.

  • Hontela A. Interrenal dysfunction in fish from contaminated sites: in vivo and in vitro assessment. Ann. Rev. Environ. Toxicol. Chem. 1998 17:44-48.

  • Kime DE, Ebrahimi M, Nysten K, Roelants I, Moore HDM, Ollevier F. Use of computer assisted sperm analysis (CASA) for monitoring the effects of pollution on sperm quality of fish: application of effects of heavy metals. Aqua. Toxicol. 1996 35: 223-237.

  • Rurangwa E, Roelants I, Huyskens G, Ebrahimi M, Kime DE, Ollevier F. The minimum acceptable spermatozoa to egg ratio for artificial insemination and the effects of heavy metals pollutants on sperm motility and fertilization ability in African catfish (Clarias gariepinus, Burchell 1822). Journal of Fish Biology. 1998 53: 402-413.

  • Laskey JW, Phelps P. Effect of cadmium and metal cations on in vivo leydig cell testosterone production. Toxicol. App. Pharmacol. 1991 108: 296-306.

  • Mukherjee D, Guha D, Kumar V. Effect of certain toxicants on gonadotropin-induced ovarian nonesterified cholesterol depletion and steroidogenic enzyme stimulation of the common carp Cyprinus carpio in vitro. Biomed. Environ. Sci. 1992 5: 92-98.

  • Mukherjee D, Kumar V, Chakraborti P. Effect of mercuric chloride and Cd chloride on gonadal function and its regulation in sexually mature common carp Cyprinus carpio. Biomed. Environ. Sci. 1994 7: 13-24.

  • Senthilkumaran B, Joy KP. Effects of photoperiod alterations on hypothalamic serotonin content and turnover, and monoamine oxidase activity in the catfish, Heteropneustes fossilis. A study correlating plasma oestradiol and gonadotrophin levels. Fish Physiol. Biochem. 1994 13: 301-307.

  • Goswami SV, Sundararaj BI. In vitro maturation and ovulation of oocytes of the catfish, Heteropneustes fossilis (Bloch): effects of mammalian hypophyseal hormones, catfish pituitary homogenate, steroid precursors and metabolites, and gonadal and adrenocortical steroids. Journal of Experimental Zoology. 1971 178: 467-478.

  • Mishra A, Joy KP. Effects of gonadotrophin in vivo and 2–hydroxyestradiol–17â in vitro on follicular steroid hormone profile associated with oocyte maturation in the catfish Heteropneustes fossilis. J. Endocrinol. 2006 189: 341-353.

  • Chaube R, Mishra S, Singh RK. In vitro effects of lead nitrate on steroid profiles in the post – vitellogenic ovary of the catfish Heteropneustes fossilis. Toxicol. In Vitro. 2010 24: 1899-1904.

  • Thomas P. Effects of arochlor 1254 and cadmium on reproductive endocrine function and ovarian growth in Atlantic croaker. Mar. Env. Res. 1989 28: 499-503.

  • Piasek M, Laskey JW. Effects of in vitro cadmium exposure on ovarian steroidogenesis in rats. J. Appl. Toxicol. 1999 19: 211-217.

  • Lizardo-Daudt HM, Bains OS, Singh CR, Kennedy CJ. Cadmium Chloride- induced disruption of testicular steroidogenesis in rainbow trout, Oncorhynchus mykiss. Arch. Of Environ. Contamin. Toxicol. 2007 55: 103-110.

  • Ebrahimi M. Effects of in vivo and in vitro zinc and cadmium treatment on sperm steroidogenesis of the African catfish Clarias gairepinus. Pak. J. Biol. Sci. 2007 10: 2862-2867.

  • Carnegie JA, Tsang BK. Follicle–stimulating hormone–regulated granulosa cell steroidogenesis: Involvement of the calcium– calmodulin system. Am. J. Obstet. Gynecol. 1983 145: 223-228.

  • Akiyama K, Sutoo D, Reid DG. A H-NMR comparison of calmodulin activation by calcium and by cadmium. Jpn. J. Pharmacol. 1990 53: 393- 401.

  • Choi MJ, Kim SC, Kim AN, Kwon HB, Ahn RS. Effects of endocrine disruptors on the oocyte maturation and ovulation in amphibians, Rana dybowskii. Integ. Biosci. 2007 11: 1-8.

  • Nishiyama S, Nakamura K, Ogawa M. Effects of heavy metals on corticosteroid production in cultured rat adrenocortical cells. Toxicol. Appl. Pharmacol. 1989 81: 174-176.

  • Stoica A, Katzenellenbogen BS, Martin MB. Activation of estrogen receptor-alpha by the heavy metal cadmium. Mol. Endocrinol. 2000 14: 545–553.

  • Szczerbik P, Mikolajczyk T, Sokolowska – Mikolajczyk M, Socha M, Chyb J, Epler P. The influence of cadmium on Prussian carp oocyte maturation, development of eggs and hatching. Czech. J. Anim. Sci. 2008 53: 36-44.

  • Nagahama Y, Yoshikuni M, Yamasita M, Nagahama Y. Regulation of oocyte maturation in fish. In Fish Physiology. Mol. Endocrinol. Fish. N. M. Sherwood, C. L. Hew, A. P. Parrell, D. J. Randall, Eds. New York. 13: 393-493; 1994.

  • Lacroix M, Hontela A. A comparative assessment of the adrenotoxic effects of cadmium in two teleost species, rainbow trout, Oncorhynchus mykiss, and yellow perch, Perca flavescens. Aquatic Toxicol. 2004 67: 13-21.

  • Sundararaj BI, Goswami SV. Hormonal regulation of in vivo and in vitro oocyte maturation in the catfish Heteropneustes fossilis (Bloch). Gen. Comp. Endocrinol. 1977 32: 17-28.


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  • In Vitro Effects of Cadmium Chloride on Steroid Profiles of Post-vitellogenic Ovary in the Catfish Heteropneustes Fossilis

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Authors

Radha Chaube
Research Scholar, Zoology Section, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi
Surabhi Mishra
Research Scholar, Zoology Section, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi
Rahul K Singh
Research Scholar, Zoology Section, Mahila Mahavidyalaya, Banaras Hindu University, Varanasi

Abstract


Cadmium (Cd) is a known endocrine disruptor with the ability to affect the production of hormones involved in the regulation of reproductive processes. The purpose of this study was to evaluate invitro effects of cadmium chloride (CdCl2) exposure on steroid levels in post-vitellogenic follicles of catfish ovary. Pieces of ovarian tissues (500mg) were incubated in culture medium in the presence or absence (control) of CdCl2 (0,0.01,0.1,1,3 and 10μg/ml) for 12 or 24hr. Estradiol-17β (E2) and testosterone were measured by ELISA. Other steroids like progestins (progesterone -P4; 17-OH-progesterone-17-P and 17, 20 β-dihydroxyprogesterone-17, 20 β-DP) and corticosteroids (cortisol, 21-deoxycortisol, corticosterone and deoxycorticosterone) were quantified by High Performance Liquid Chromatography. The results show that Cd produced biphasic effects on E2, T, P4, 17,20β-DP, cortisol, deoxycorticosterone, and 21-deoxycortisol, stimulatory at lower concentrations and inhibitory at higher concentrations. In contrast, 17-P and corticosterone were inhibited in a dose-dependent manner. Thus, Cd can influence ovarian steroidogenesis adversely, affecting gametogenesis and ovulation resulting in the decline of fish population.

Keywords


Cadmium, Ovary, Invitro Steroid Levels, Endocrine Disruptors, Catfish

References