Indian Journal of Public Health Research & Development

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Differentiation of Bone and Soft Tissue Using Methylene Blue - Acid Fuchsin: a New Stain Combination


Affiliations
1 Department of Oral Pathology, KLE VK Institute of Dental Sciences, KLE University, Nehru Nagar, Belgaum-590010, Karnataka, India
2 Department of Oral Pathology, Bharatiya Vidhyapeeth Dental College, Sangli, Maharashtra, India
3 Dept. of Pathology, JN Medical College, Belgaum-10, India
4 Department of Oral Pathology, ITS Dental College & Hospital, Noida, India
     

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Introduction

Hematoxylin-Eosin(H-E) provides optimal staining for histology, but in formalin-fixed, decalcified, paraffinembedded bone tissue, both bone and soft tissue appear the same shade of pink. In addition to the improved staining properties, the methylene blue-acid fuchsin stain is also superior to H-E in terms of its stability, cost and ease of use.

Aim & Objectives

To determine the efficacy of methylene blue-acid fuchsin stain combination to differentiate mineralized structures from surrounding soft tissues in various oral lesions.

Material & Methods

Two sections each of peripheral cemento-ossifying fibroma, central cemento-ossifying fibroma, fibroma with calcification, fibrous dysplasia, adenomatoid odontogenic tumour and pleomorphic adenoma were taken. One section was stained with hematoxylin-eosin and the other with methylene blue-acid fuchsin.

Results & Conclusion

In contrast to standard hematoxylin-eosin stain, which stains both bone and soft tissues pink, the methylene blue-acid fuchsin stain combination demonstrates remarkable contrast with bone appearing bright pink and the soft tissue blue-purple.


Keywords

Methylene Blue, Acid Fuchsin, Bone, Soft Tissue
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  • Barlet JP, Coxam V, Davicco MJ, Gaumet N: Animal models of post-menopausal osteoporosis. Reprod Nutr Dev 1994; 34: 221-236

  • Keffer J et al: Transgenic mice expressing human tumor necrosis factor: A predictive genetic model of arthritis. Embo J 1991; 10: 4025-4031

  • Schwarz EM et al. Quantitative small animal surrogate to evaluate drug efficacy in preventing wear debris induced osteolysis. J Orthop Res 2000; 18: 849-855

  • Rider KA, Flick LM. Differentiation of bone and soft tissues in formalin fixed, paraffin embedded tissue by using methylene blue/ acid fuchsin stain. Ana Quant Cytol Histo 2004; 26 (5): 246-248


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  • Differentiation of Bone and Soft Tissue Using Methylene Blue - Acid Fuchsin: a New Stain Combination

Abstract Views: 265  |  PDF Views: 0

Authors

Veena V. Naik
Department of Oral Pathology, KLE VK Institute of Dental Sciences, KLE University, Nehru Nagar, Belgaum-590010, Karnataka, India
Pallavi D. Shirol
Department of Oral Pathology, Bharatiya Vidhyapeeth Dental College, Sangli, Maharashtra, India
Sunita Patil
Dept. of Pathology, JN Medical College, Belgaum-10, India
N. Mishra Mithilesh
Department of Oral Pathology, ITS Dental College & Hospital, Noida, India

Abstract


Introduction

Hematoxylin-Eosin(H-E) provides optimal staining for histology, but in formalin-fixed, decalcified, paraffinembedded bone tissue, both bone and soft tissue appear the same shade of pink. In addition to the improved staining properties, the methylene blue-acid fuchsin stain is also superior to H-E in terms of its stability, cost and ease of use.

Aim & Objectives

To determine the efficacy of methylene blue-acid fuchsin stain combination to differentiate mineralized structures from surrounding soft tissues in various oral lesions.

Material & Methods

Two sections each of peripheral cemento-ossifying fibroma, central cemento-ossifying fibroma, fibroma with calcification, fibrous dysplasia, adenomatoid odontogenic tumour and pleomorphic adenoma were taken. One section was stained with hematoxylin-eosin and the other with methylene blue-acid fuchsin.

Results & Conclusion

In contrast to standard hematoxylin-eosin stain, which stains both bone and soft tissues pink, the methylene blue-acid fuchsin stain combination demonstrates remarkable contrast with bone appearing bright pink and the soft tissue blue-purple.


Keywords


Methylene Blue, Acid Fuchsin, Bone, Soft Tissue

References