Indian Journal of Public Health Research & Development

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Comparison of Hair Follicle and Skin for Ante Mortem Detection of Rabies: a Molecular Approach


Affiliations
1 Department of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
2 School of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
     

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Background: Rabies is an enzootic, fatal disease and early detection of this disease is necessary. Till now a reliable diagnosis is possible only after death of animal.

Objectives: So the present study was undertaken to use the molecular approaches like nested RTPCR and SYBR Green real time PCR for early diagnosis of rabies in suspected animals using less invasive non neural tissues like skin biopsy and hair follicles.

Method: 12 cases of rabies suspected live animals were presented to the Veterinary Clinics, GADVASU, Ludhiana, India.

Results: The sensitivity of nested RT-PCR for hair follicles and skin samples was 50% and 57.1% respectively. A sensitivity of 62.5% and 71.4% was observed with real time PCR in hair follicles and skin samples.

Conclusion: We suggest that non-invasive molecular approaches may be used for the early and accurate diagnosis of rabies in animals.


Keywords

Diagnosis, Hair Follicle, Skin, Rabies
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  • Comparison of Hair Follicle and Skin for Ante Mortem Detection of Rabies: a Molecular Approach

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Authors

Ambika Kaw
Department of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
C. K. Singh
Department of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
Ramneek
School of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
D. Deka
School of Animal Biotechnology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
B. S. Sandhu
Department of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
S. Awahan
Department of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India
N. K. Sood
Department of Veterinary Pathology, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana, India

Abstract


Background: Rabies is an enzootic, fatal disease and early detection of this disease is necessary. Till now a reliable diagnosis is possible only after death of animal.

Objectives: So the present study was undertaken to use the molecular approaches like nested RTPCR and SYBR Green real time PCR for early diagnosis of rabies in suspected animals using less invasive non neural tissues like skin biopsy and hair follicles.

Method: 12 cases of rabies suspected live animals were presented to the Veterinary Clinics, GADVASU, Ludhiana, India.

Results: The sensitivity of nested RT-PCR for hair follicles and skin samples was 50% and 57.1% respectively. A sensitivity of 62.5% and 71.4% was observed with real time PCR in hair follicles and skin samples.

Conclusion: We suggest that non-invasive molecular approaches may be used for the early and accurate diagnosis of rabies in animals.


Keywords


Diagnosis, Hair Follicle, Skin, Rabies