International Journal of Pharmaceutical Sciences and Nanotechnology (IJPSN)

The PDF file you selected should load here if your Web browser has a PDF reader plug-in installed (for example, a recent version of Adobe Acrobat Reader).

If you would like more information about how to print, save, and work with PDFs, Highwire Press provides a helpful Frequently Asked Questions about PDFs.

Alternatively, you can download the PDF file directly to your computer, from where it can be opened using a PDF reader. To download the PDF, click the Download link above.

Fullscreen Fullscreen Off


Tolterodine is used to treat overactive bladder symptoms such as loss of bladder control (incontinence) or a frequent need to urinate. A simple and inexpensive method was developed with high performance liquid chromatography with PDA detection for determination of tolterodine and related impurities. The chromatographic separations were achieved on (250 × 4.6 mm), 5.0 μm make: symmetry, C8 column employing methanol and 0.1% orthophosphoric acid in water, in the ratio of 90:10 as mobile phase with gradient programmed at flow rate 1.0 mL/min was chosen. Three impurities were eluted within 35 minutes. The column temperature was maintained at 35o C and a detector wavelength of 220 nm was employed. The method was successfully validated by establishing system suitability, specificity, linearity, accuracy, LOD and LOQ. The validated method is useful for tolterodine assay in pharmaceutical products.

Keywords

HPLC; Method validation; Tolterodine; LOD and LOQ.
User
Notifications
Font Size