Journal of Scientific and Industrial Research

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Enhanced Degradation of C17 and C34 of Bonny Light Crude Oil by Enterobacter sp.


Affiliations
1 Department of Microbiology, University of Calabar, India

Investigation of enhanced potential of Enterobacter sp. in biodegradation of Bonny light crude oil components, C17 and C34 was carried out. Samples of water and sediment were collected from predetermined locations and subjected to microbiological analyses using standard methodologies. Of the 32 potential hydrocarbon-utilizing bacteria isolated from the water and sediment samples that were exposed to crude oil contamination, only one isolate,Enterobacter sp. (Y8ESWS3) was found to effectively biodegrade 99.8% of C17 and 93% of C34 of the aliphatic hydrocarbon after 28 days of degradation. Analysis of variance assessed by gas chromatography revealed that bacterial species (P=9.05E-05<0.05) and nature of hydrocarbon fraction (P=8.55E-08<0.05) significantly influence the hydrocarbonoclastic potential. The high growth rate of Enterobacter sp. and its ability to considerably reduce the hydrocarbon component, C17 and C34 of Bonny light crude oil makes it, an impressive candidate for consideration in bio –augmentation.
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  • Enhanced Degradation of C17 and C34 of Bonny Light Crude Oil by Enterobacter sp.

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Authors

Abigail Johnny Nkanang
Department of Microbiology, University of Calabar, India
Sylvester Peter Antai
Department of Microbiology, University of Calabar, India
Atim David Asitok
Department of Microbiology, University of Calabar, India

Abstract


Investigation of enhanced potential of Enterobacter sp. in biodegradation of Bonny light crude oil components, C17 and C34 was carried out. Samples of water and sediment were collected from predetermined locations and subjected to microbiological analyses using standard methodologies. Of the 32 potential hydrocarbon-utilizing bacteria isolated from the water and sediment samples that were exposed to crude oil contamination, only one isolate,Enterobacter sp. (Y8ESWS3) was found to effectively biodegrade 99.8% of C17 and 93% of C34 of the aliphatic hydrocarbon after 28 days of degradation. Analysis of variance assessed by gas chromatography revealed that bacterial species (P=9.05E-05<0.05) and nature of hydrocarbon fraction (P=8.55E-08<0.05) significantly influence the hydrocarbonoclastic potential. The high growth rate of Enterobacter sp. and its ability to considerably reduce the hydrocarbon component, C17 and C34 of Bonny light crude oil makes it, an impressive candidate for consideration in bio –augmentation.