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Purification, Characterization and Application Study of Bacterial Tannase for Optimization of Gallic acid Synthesis from Fruit Waste


Affiliations
1 University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali 140 413, Punjab, India
2 School of Engineering & Technology, Sharda University, Greater Noida 201 306, UP, India
 

Tannase produced extracellularly by the bacterial strain Bacillus haynesii SSRY4 MN031245 was purified in step-wise manner through ammonium sulphate precipitation, dialysis, followed by anion exchange chromatography. Tannase was purified to 42.0-fold with 36.30% enzyme yield. The enzyme was relatively stable from 30 to 50° and pH (4.0–6.0) for up to 4 hours. Partially purified tannase (16.80 U/ml) was able to synthesize 20.304 mg/ml gallic acid from the fruit waste under optimized conditions. The results of application study suggest that bacterial tannase could provide a new source for Gallic acid synthesis from the fruit waste for industrial applications. Our research findings could provide a value chain to fruit waste and help in reducing the waste generation from fruit processing industries.

Keywords

Bacillus haynesii, Enzyme, Hydrolysis, Quantification, Yield.
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  • Purification, Characterization and Application Study of Bacterial Tannase for Optimization of Gallic acid Synthesis from Fruit Waste

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Authors

Sunny Dhiman
University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali 140 413, Punjab, India
Gunjan Mukherjee
University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali 140 413, Punjab, India
Anu Kumar
University Institute of Biotechnology, Chandigarh University, Gharuan, Mohali 140 413, Punjab, India
Rita Singh Majumdar
School of Engineering & Technology, Sharda University, Greater Noida 201 306, UP, India

Abstract


Tannase produced extracellularly by the bacterial strain Bacillus haynesii SSRY4 MN031245 was purified in step-wise manner through ammonium sulphate precipitation, dialysis, followed by anion exchange chromatography. Tannase was purified to 42.0-fold with 36.30% enzyme yield. The enzyme was relatively stable from 30 to 50° and pH (4.0–6.0) for up to 4 hours. Partially purified tannase (16.80 U/ml) was able to synthesize 20.304 mg/ml gallic acid from the fruit waste under optimized conditions. The results of application study suggest that bacterial tannase could provide a new source for Gallic acid synthesis from the fruit waste for industrial applications. Our research findings could provide a value chain to fruit waste and help in reducing the waste generation from fruit processing industries.

Keywords


Bacillus haynesii, Enzyme, Hydrolysis, Quantification, Yield.

References