A B C D E F G H I J K L M N O P Q R S T U V W X Y Z All
Paul, Goutam
- A Bio-Technological Approach on Removal of Lead Ions from Aqueous System by Dry Cells of Pseudomonas Aeruginosa DSGPM4 Species
Authors
1 Environmental Physiology division, Microbiology unit, Dept. Of Physiology, University Of Kalyani, Nadia, West Bengal, Pin-712435
2 Dept of Physiology, University of Kalyani, Nadia, West Bengal, Pin-712435
Source
International Journal of Biotechnology and Bioengineering Research, Vol 4, No 1 (2013), Pagination: 11-19Abstract
The pollution of the environment with toxic heavy metals is spreading throughout the world along with industrial progress. The bioremediation of heavy metals using microorganisms has received a great deal of attention in recent years, not only as a scientific novelty but also for its potential application in industry. The role of microorganisms in bioremediation is important because of their ability to degrade hazardous compounds into harmless ones. Copper, nickel, cadmium, lead, chromium are known to be the most commonly heavy metals and wide spread contaminants of the environment. The removal of lead ion from the aqueous solution under different experimental conditions by using dry cells of Pseudomonas aeruginosa DSGPM4 sp [GenBank Accn JN245880No] was investigated in this study. The lead uptake was dependent on initial pH and initial copper concentration, with pH 5.0 being the optimum value. At various initial lead concentrations (100-300 ppm), biosorption equilibrium was attended after 320 mint(s) incubation.The biomass concentration also influenced the biosorption efficiency. In this study biomass concentration was used 300mg/50ml to 2400mg/50ml. Maximum uptake was achieved by 2400mg/50ml of biomass concentration. It was also found that increase in the biomass concentration, removal percentage increases, but the specific uptake (q value) decreases. Other parameter also influenced the lead biosorption efficiency, such as temperature. Optimum temperature was found in this study was 40°C. This result showed that the biomass of Pseudomonas aeruginosa DSGPM4 sp is a suitable biosorbent agent for the removal of lead from aqueous solution. Further investigation for molecular characterization of this bacterium is needed.Keywords
Biosorption, Heavy Metal, Sorption EquilibriumReferences
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- Sannasi P, Kader J, Ismail BS, Salmijah, S (2006); Sorption of Cr (VI), Cu(II) and Pb(II0 by growing and non-growing cells of a bacterial consortium. Bioresour. Technol., 97, 740-747.
- Banjerdkij P., vattanaviboon P., Mongkolsuk S., (2005); Exposure to cadmium elevates expression of genes in the oxy R and Ohr R regulations and induces cros-resistance to peroxide killing treatment in Xanthomonas campestris. Appl.environ. Microbiol., 71(4): 1843-1849.
- Say R, Denizli A, Arica MY (2001) ; Biosorption of cadmium (II), lead (II) and copper (II) with the filamentous fungus Phanerochaete chrysosporium. Bioresource Technol. 76: 67-70.
- Yan G, Viraraghavan T (2000); Effect of pretreatment on the bioadsorption of heavy metals on Mucor rouxii.Water SA 26: 119-123.
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- Molecular Characterization of Metal and Antibiotic Resistance Activities in a Bacterial Population Isolated from Waste Water Sample
Authors
1 PG Dept of Biotechnology, Acharya’s Bangalore B- school (ABBS), Lingerdhnahalli, Magadi Road, Bangalore-91
2 Dept of Physiology, University of Kalyani, Nadia, West Bengal, Pin-712435
Source
International Journal of Biotechnology and Bioengineering Research, Vol 4, No 1 (2013), Pagination: 21-30Abstract
The incidence of multiple metal and antibiotic resistance among bacterial populations poses a potential threat to human health. The co-existence of metal and antibiotic resistance in bacterial strains suggests the role of metals as a factor, which can also contribute to such a phenomenon. The objective of this study was to characterize multiple metal and antibiotic resistance bacteria from the water samples of a main drainage system of water purifying plant in Bangaluru, Karnataka and to transform the plasmid DNA into another species to confirm about their horizontal gene transfer. A total of 5 strains designated as DSGPM1-5 [3 are Gram- negative bacteria (GenBank Accn No JN228116, JN245880 and JN245881 respectively) and 2 are Gram-positive bacteria] were identified by physico-chemical methods. All the 5 bacterial isolates showed different antibiotic resistance activity (Ampicillin, Chloramphenicol, Erythromycin, Streptomycin, Tetracyclin). All the isolates showed different metal resistance activity too (Zn2+,Mn2+, Ni2+, Cd2+ ,Cu2+ ,Hg2+ , Pb2+), but among them only two isolate (DSGPM3 and DSGPM4) showed mercury resistance activity. These strains were screened to find the presence of an endogenous plasmid DNA. One Gram-negative strain (Bacillus sp.) DSGPM4 was found to have a plasmid DNA of different restriction cutting site and that could replicate and express in other Gram-negative bacteria (E.coli DH5α).Thus, from our result it can be concluded that multiple metal and antibiotic resistance among bacterial populations probably through horizontal gene transfer. The transformed DH5α-DSGPM4 showed increase MIC values for metals, 5 fold for Zn2+, 4 fold of Mn2+ and 2 fold of Hg2+, where as for Ni2+, Cd2+ and Pb2+ the MIC values were not significantly changed after the transformation. This was the prove that this plasmid may have potential applications in molecular biology for cloning and expression of foreign genes.Keywords
Metal Resistance, Metal Pollution, Antibiotic, TransformReferences
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- Alonso A, Sanchez P et al. (2001); Environmental selection of antibiotic resistance genes.Environ,Microbial.3:1-9.
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- Optimization of Bacterial Extra-Cellular Polymeric Substance Production and Application of this Exopolymer in Toxic Metals Biosorption from Aqueous System
Authors
1 PG Dept of Biotechnology, Acharya’s Bangalore B - School (ABBS), Lingerdhinahalli, Magdi Road, Bangalore-560091, IN
2 Dept of Physiology, University of Kalyani, Nadia, West Bengal, Pin-741235, IN
Source
International Journals of Biotechnology and Biochemistry, Vol 9, No 1 (2013), Pagination: 13-21Abstract
Extra- cellular polymeric substances (EPS) of bacteria play a very important role in absorption of heavy metals. The aim of this study was to optimize the production of EPS and study the role of EPS in biosorption of Cu+2, Cd+2 and Ni+2 in aqueous phase. A total of five bacterial strain were isolated, identified and designated them as DSGPM1 to DSGPM5. All of them had different metal resistance capacity. Among these five strains, it was found that strain DSGPM4 produced high polymer in presence of 1% sucrose and 4% glycerol in nutrient broth (8.4mg/ml). The optimum condition was found for production of maximum polymer at 370C, pH 7.0, 200 rpm shaking during 4 days in presence of 1% sucrose and 4% glycerol in media. In regard to metal removal by EPS, it was found that polymer bio-absorb metals more than dry cells. The biosorption of Cu+2, Cd+2 and Ni+2 were found by polysaccharide as 2.21 mg/gm, 2.625 mg/gm and 2.295mg/gm respectively. However, the whole cells only biosorbed these metals (Cu+2, Cd+2 & Ni+2) by 1.71mg/gm, 2.15 mg/gm and 1.765 gm of dry cells respectively. Our results could establish a basis for evaluating the role of bacteria in the search for an environmentally friendly approach to dealing with heavy metal pollutants in aqueous phase.Keywords
Extracellular Polymeric Substances, Absorption, Aqueous Phase, Dry CellsReferences
- Rehman A,Shakoori FR,et al.(2007); Multiple metal resistance and uptake by a ciliate,Stylomychia mytilus,isolated from industrial effluents and its possible use in wastewater treatment Bull,Environ.Contam.Toxicol,79:410-414.
- Alonso A, Sanchez P et al. (2001); Environmental selection of antibiotic resistance genes.Environ,Microbial.3:1-9.
- Austin CB, Wright MS, et al(2006); Co-selection of antibiotics and metal resistance.Trends Microbial.14:176-182.
- Summers AO, (2002); Generally overlooked fundamentals of bacterial genetics and ecology.Chin.Infect.Dis.34: S85-S92.
- Summers AO,Wireman J et al.(1993); Mercury released from dental silver fillings provokes an increase in mercury-resistant and antibiotic resistant bacteria in oral and intestinal floras of primates. Antimicrob Agents Chemother.37:825-834.
- Krulwich TA, Lewinson O. et al.(2005); Do physiological roles foster persistence of drug/multidrug-efflux transporter?A case study,Nat,Rev.Microbiol.3:566-572.
- Veglio F, Boelchini F (1997); Removal of heavy metals by biosorption. A review. Hydrometallurgy. 44: 301-316.
- Sannasi P, Kader J, Ismail BS, Salmijah, S (2006); Sorption of Cr (VI), Cu(II) and Pb(II) by growing and non-growing cells of a bacterial consortium. Bioresour. Technol., 97, 740-747.
- Say R, Denizli A, Arica MY (2001) ; Biosorption of cadmium (II), lead (II) and copper (II) with the filamentous fungus Phanerochaete chrysosporium. Bioresource Technol. 76: 67-70.
- Yan G, Viraraghavan T (2000); Effect of pretreatment on the bioadsorption of heavy metals on Mucor rouxii.Water SA 26: 119-123.
- Sponer J, Burda JV, Sabat M, Leszczynski J, Hobza P (1998); Intraction between the guanine-cytosine Watson-Crick DNA base pair and hydrated group lia (Mg+2, Ca+2, Sr+2 , Ba+2 ) and group lib (Zn+2, Cd+2, Hg+2) metal cations . J. Phys. Chem. A. 102: 5951-5957.
- Optimization of Protease Enzyme Production From Heavy Metal and Antibiotic Resistant Bacillus Species Isolated from Waste Water Sample
Authors
1 PG Dept of Biotechnology, Acharya’s Bangalore B- school (ABBS), Lingerdhnahalli, Magadi Road, Bangalore-560091, IN
2 Environmental Physiology Division, Microbiology Unit, Dept of Physiology, University of Kalyani, Nadia, West Bengal, Pin-712435, IN
Source
International Journals of Biotechnology and Biochemistry, Vol 9, No 1 (2013), Pagination: 49-59Abstract
The bacterial isolate Bacillus sp DSGPM3, having heavy metals and antibiotic resistant activity, isolated from waste water and screened for protease enzyme production. On the basis of cultural, morphological, physiological and biochemical characteristics the isolate Bacillus sp DSGPM3 was identified (NCBI Accn No JN228116).Among the different carbon sources, cellobiose was found to be best carbon source. Yeast extract was found to the optimum nitrogen source for protease enzyme production. A temperature of 370C and pH 7.5 were found optimum to induce the protease production of the isolate and showed highest proteolytic activity. This protease enzyme has huge application in various industries.Keywords
Bacillus sp, Protease Enzyme, Proteolytic Activity.References
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- Role of Physical Activity on Calorie-Induced Changes in Cognition and Depression of Young and Aged Humans
Authors
1 Department of Biochemistry, University of Calcutta, Kolkata - 700 019, IN
2 Department of Physiology, University of Kalyani, Kalyani, Nadia-741 235, West Bengal, IN
Source
The Indian Journal of Nutrition and Dietetics, Vol 54, No 3 (2017), Pagination: 316-335Abstract
Normal aging is known to encounter stress. Association of aging with declining cognition and elevated depression is a phenomenon which can be modulated by lifestyle. Caloric restriction down regulates normal aging by lowering stress and has been found to protect aging processes. The present study assessed the effect of Calorie Restriction (CR) and Physical Activity (PA) on aging-induced changes on cognition and depression. Healthy male aged (20-35 years; N=312) and (55-70 years; N=392) were considered from low income and middle education population. Data related to socioeconomic status, health status, cognitive performance by mini-cog and 6CIT, depression by CES-D Scale, PA Level (PAL) and calorie intake were collected through questionnaires. AP (at-par) diet (amount consumed equivalent to the amount of energy required for 24 h) reduced cognitive function of the aged group compared to the young of the same calorie group without affecting their depression under similar conditions. CR diet improved cognition with a lowering of depression in the young subjects compared to aged of the same group. High PAL in comparison to low PAL irrespective of age and calorie intake positively modulated cognition and depression of the subjects. These results suggest that (a) young population with CR diet and aged population with AP diet are benefitted in both cognitive ability and depression (b) high PAL unlike low PAL improved both cognition and depression of both young and aged population irrespective of their calorie intake.Keywords
Aging, Calorie Restriction, Cognitive Function, Depression, Physical Activity.References
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- Nature of Solid Wastes and Its Management Problem in Burdwan Town, West Bengal
Authors
1 Department of Environmental Science, The University of Burdwan, Burdwan, West Bengal, 713104, IN
Source
Indian Science Cruiser, Vol 24, No 4 (2010), Pagination: 26-34Abstract
Solid waste management poses crisis in many of the world's large urban areas due to explosive population growth and massive shift of population towards urban. Keeping in mind of the above problem a study has been done to find out the possible sources and the nature of solid wastes generated at different places of Burdwan town, West Bengal. Moreover, a questionnaire was made and was applied the same to the locality adjacent to the dumping centers. It was found that solid wastes mainly are polythene packets, plastic bottles, glass bottles, papers, coal ash, cottons, bandages, injection syringes, jute packets, needles, sands, torn cloths, torn mats, torn sacks, clay pots, scales of fish, rotten vegetable etc. The physicochemical parameter of the solid wastes viz. pH, phosphate, moisture, potassium, organic matter, organic carbon, nitrogen were assessed. The C/N ratio are 7.66-8.41; 0.029- 0.144,21.88-40.67; 0.54-0.67; 6.311-10.976; 3.406-7.011; 0.57 and 5.01-10.11 respectively. The pH of drinking water collected from different distance viz. (5 m, 10 m, 15 m, 20 m) from dumping centers does not show significant difference and ranged between 6.5-6.8.