Informatics Publishing Limited

J. Madhusudhanan ¹, K. Sathishkumar ²

Affiliations
1 Department of Biotechnology, Shri Andal Alagar College of Engineering (SAACE), Chennai-603111, IN
2 Department of Chemical Engineering, Sri Sivasubramaniya Nadar College of Engineering (SSNCE), Chennai-603110, IN

Abstract

New biologic drugs such as proteins and nucleic acids require novel delivery technologies that will minimize side effects and lead to better patient compliance. Hence, various research using Nanoparticle as carrier has been done for the delivery of gene, drug, photo acoustic imaging and LASER induced photo thermal therapy. In our research, we have studied the delivery of an enzyme using nanoparticles as a carrier in microorganism. The enzyme which is capable of cleaving the peptides is made inactive by inhibitors present inside the cell. But we have studied that the enzyme can be made resistive by coating with nanoparticles. The action of this enzyme has been analyzed on a specific enzyme. It has been selected as target because one enzyme can't cleave other enzyme in active form but its peptides can be cleaved inside the cell alone. In this study, we have used complex system of an enzyme and nanoparticles to cleave the enzyme inside the cell which shows the successful delivery of the protein. In addition, by combining with nanoparticles, the intracellular stability of the enzyme can be maintained. These results suggest that a protein or drug can be conjugated with nanoparticles against a specific disease.

Keywords

Nanoparticles, Intra Cellular Enzyme, Extracellular Enzyme, Protein Assay.

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T. Anitha ¹, K. Sathishkumar ²

Affiliations
1 Rajalakshmi Engineering College, Thandalam, Chennai, IN
2 SSN College of Engineering, Kalavakkam, Chennai, IN

Abstract

The contamination of soil and water by metals all over the world continues to pose a serious danger to the environment and human health. Chitosan is a well-known and efficient metal chelator, but its practical use is limited due to the relatively high costs of constructing clean-up devices (filters) from chitosan alone. Chitosan coated sand (CCS) was used as an adsorbent for the adsorption of chromium metal ions. The various physical parameters like adsorbate concentration, adsorbent dose, contact time have been studied. The maximum percentage of adsorption of chromium was found for chitosan, CCS and sand, and the maximum adsorption was obtained for the system CCS. The SEM images of chitosan and chitosan coated sand were studied. The possible recovery and reuse of the adsorbent material was done by recovering chromium using desorption studies. The possibility of using chitosan-coated sand to build inexpensive large-scale barrier filters for metal removal from moving contaminated groundwater plumes was found to be effective.

Keywords

Chitosan, SEM, Isotherm, Chromium, CCS.

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M. Sibiram , V. Balaji , K. Sathishkumar , P. Vignesh

Abstract

The widespread use of electronic equipments and their integration into the vehicular system have become a necessity in the automobile industry. Automation of vehicles are being carried out with the help of Embedded Systems. In our project, we have proposed and completed four objectives in automotives. The automobiles basically includes wagon under wheels which helps in transportation, locomotion, etc., and reducing human efforts. Existing luxury vehicles have certain disadvantages considering the tyre pressure, accurate quantity of fuels, alternator in which the internal component damage will not be notified and finally a battery notification which will not come with a backup battery. Hence our project idea is to set separate detecting digital meters and controlling equipments for all above said problems inside automobiles itself. This will improve the reliability of the machines used and also avoids accidents, at the end consumer level.

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R. Parkavi ¹, G. Madhan ², K. Srinivasan ², K. Sathishkumar ³, A. Chandramohan ⁴, K. Dinakaran ¹

Affiliations
1 Department of Chemistry, Thiruvalluvar University, Vellore - 632115, India, IN
2 Department of Chemistry, Thiruvalluvar University, Vellore - 632115, India., IN
3 Department of Chemical Engineering, Sri Sivasubramaniya Nadar College of Engineering, Kalavakkam, Chennai - 603110, India .i, IN
4 Department of Chemical Engineering, Sri Sivasubramaniya Nadar College of Engineering, Kalavakkam, Chennai - 603110, India., IN

Abstract

We have synthesized new fluorescent organic molecules namely arylidene malanonitriles, through Knovenegal condensation reaction of aryl aldehydes and malanonitrile, which are capable of detectingCd2+and Cu2+ ions in water with sensitivity. The synthesized molecules are characterized through infrared spectrometry, high resolution Mass spectrometry and Nuclear Magnetic Resonance spectroscopy. The fluorescent organic molecules exhibited a fluorescent emission and significant UV-Vis absorption, the intensity of which is increased proportional to the addition of Cd2+ and Cu2+ ions. The lowest detection limit for the Cd2+and Cu2+ were found to be 2.0×10-10 M and 4×10-12 M, respectively.

Keywords

Knovenegal reaction, Malanonitrile, Fluorescent sensor, Photoluminescence, Heavy metal detection.

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