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Vaithiyanathan, Veluchamy
- Assessment of Antioxidant Potential and Acute Toxicity Studies of Whole Plant Extract of Pergularia daemia (Forsk)
Abstract Views :191 |
PDF Views:1
Authors
Affiliations
1 Department of Biochemistry and Biotechnology, Annnamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu, IN
1 Department of Biochemistry and Biotechnology, Annnamalai University, Annamalai Nagar, Chidambaram, Tamil Nadu, IN
Source
Toxicology International (Formerly Indian Journal of Toxicology), Vol 22, No 1 (2015), Pagination: 54-60Abstract
Background: Pergularia daemia (Asclepiadacea) is a fetid- smelling perennial herb growing well along the river bang and road sides of India. Naturally the plant has powerful antioxidants including polyphenols, flavanoids, steroids and terpenoids. Objective: The aim of this study is to evaluate the in vitro antioxidant potential and to determine the median lethal dose (LD50) of crude ethyl acetate and methanol extracts of Pergularia daemia. The plant Pergularia daemia possess effective scavenging activity against 2, 2’ azino bis (3 ethylbenzothiazoline 6 sulfonic acid (ABTS), nitric oxide and reducing power radicals at different concentrations (100, 200, 300, 400 & 500 μg/mL) of both extracts. Results: From the above in vitro assay we have exposed that the methanolic extract exert higher antioxidant activity at 400 μg/mL than ethyl acetate extract. Acute toxicity study revealed that the extracts showed no signs of toxicity upto a dose level of 2500 mg/kg b.wt. Conclusion: Thus our findings provide that both extracts of Pergularia daemia possess a strong antioxidant capacity and are relatively has high margin of safety.Keywords
Acute Toxicity (LD50), in Vitro Antioxidants, Pergularia daemia, Phytochemical Screening.
- Antiproliferative Effect of Alpha Amyrin on Hep2 Cells by Inducing Cytotoxicity and Oxidant Antioxidant Status Modifications
Abstract Views :618 |
PDF Views:354
Authors
Affiliations
1 Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar - 608 002, Tamil Nadu, IN
1 Department of Biochemistry and Biotechnology, Annamalai University, Annamalai Nagar - 608 002, Tamil Nadu, IN
Source
FoodSci: Indian Journal of Research in Food Science and Nutrition, Vol 3, No 2 (2016), Pagination: 44-47Abstract
Alpha amyrin, a plant bioactive triterpenoids, present in higher plants and particularly in Pergularia daemia. The compound alpha amyrin is a potent antioxidant, anti-inflammatory and antinociceptive, antipruritic, gastro protective and hepatoprotective agent. The anticancer effect of alpha amyrin has not been examined in human laryngeal cancer model. So this study evaluated its potential on Hep2 cells and anti-oxidant modifications. The current investigations explored the alpha amyrin possessed excellent manner (10, 20, 40, 80,160 and 320) μmol/ml and the IC50 value was found to be 69.32 μmol/ml. The TBARS level increased and antioxidant levels decreased in alpha amyrin treated Hep2 cells as compare to untreated cancer Hep2 cells. On the basis of our result alpha amyrin possess excellent antiproliferative ability on Hep2 cells by inducing cytotoxicity as well as oxidant antioxidant alterations. Therefore, alpha amyrin has therapeutic potential on various types of cancer.Keywords
Alpha Amyrin, Anti-Oxidant, Laryngeal Cancer, Pergularia Daemia, Triterpenoids.Full Text
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