Indian Journal of Science and Technology

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A Study on the Optimum Condition of Haematoxylin- Eosin using RGB Analysis


Affiliations
1 Department of Pathology, Eulji University Hospital, Korea, Republic of
2 Department of Clinical Laboratory Science Daejeon Health Institute of Technology, Korea, Republic of
3 Department of Physical Therapy, SunMoon University, Korea, Republic of
 

Objectives: This research was conducted to look for the vest conditions of Hematoxylin-eosin staining. Methods/ Statistical Analysis: Method was investigated the Hematoxylin-eosin staining, Microscopy and RGB analysis. Findings: The result was as follows. The thickness of the optimal condition is 3 cm, the time of the optimal Hematoxylin 4 minutes 30 seconds and eosin 3 minutes 45 seconds. Also, RGB analysis was green. So green showed a significant difference in the change width to each stage, and the biggest change was 52.74 at 2㎛ and 3㎛. Improvements: Therefore, an appropriate fixed time is needed to be found to get staining intensity and sharpness, and the freshness of reagent for each stage should be maintained.

Keywords

Eosin, Hematoxylin, RGB, Microscopy, Stain.
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  • A Study on the Optimum Condition of Haematoxylin- Eosin using RGB Analysis

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Authors

Jong Ho Back
Department of Pathology, Eulji University Hospital, Korea, Republic of
Jean Soo Kim
Department of Clinical Laboratory Science Daejeon Health Institute of Technology, Korea, Republic of
Dong Yeop Lee
Department of Physical Therapy, SunMoon University, Korea, Republic of

Abstract


Objectives: This research was conducted to look for the vest conditions of Hematoxylin-eosin staining. Methods/ Statistical Analysis: Method was investigated the Hematoxylin-eosin staining, Microscopy and RGB analysis. Findings: The result was as follows. The thickness of the optimal condition is 3 cm, the time of the optimal Hematoxylin 4 minutes 30 seconds and eosin 3 minutes 45 seconds. Also, RGB analysis was green. So green showed a significant difference in the change width to each stage, and the biggest change was 52.74 at 2㎛ and 3㎛. Improvements: Therefore, an appropriate fixed time is needed to be found to get staining intensity and sharpness, and the freshness of reagent for each stage should be maintained.

Keywords


Eosin, Hematoxylin, RGB, Microscopy, Stain.



DOI: https://doi.org/10.17485/ijst%2F2016%2Fv9i40%2F125778