Indian Journal of Science and Technology

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Clinical Evaluation of Automated BACTEC MGIT 960 System for Identification, Recovery and Drug Susceptibility Testing of Mycobacterium tuberculosis Clinical Isolates


Affiliations
1 Department of Microbiology, Intermediate Reference Laboratory, State TB Training and Demonstration Centre, Government Hospital for Chest Diseases, Gorimedu, Puducherry - 605006, India
2 Department of Biotechnology, Sathyabama University, Rajiv Gandhi Road, Jeppiaar Nagar, Chennai - 600119, Tamilnadu, India
 

Background and Objectives: The objective of this study was to evaluate the clinical applicability of BACTEC Mycobacterial Growth Indicator Tube 960TM system with Lowenstein-Jensen (LJ) culture for routine recovery and drug susceptibility test analysis of mycobacteria from clinical specimens. Method and Statistical Analysis: One hundred and seven clinical specimens were processed by routine laboratory procedures like p-nitro benzoic acid test and PCR amplification of IS6110 and inoculated in LJ medium and MGIT for mycobacterial growth recovery and DST procedures. Findings: Ninety four (87.8%) samples were demonstrated reproducible result by MGIT, in which 89 (83.1%) were smear positive and all the specimens were confirmed as M. tuberculosis complex by MGIT PNB and IS6110 PCR procedures. The observed mean time for mycobacterial detection was 9 days for primary culture and 11 days for DST in MGIT system. Applications/Improvements: The use of BACTEC MGIT 960 system was found to be a sensitive, rapid mycobacterial recovery and culture system and offers precise identification and detection of drug resistance from clinical isolates at earliest.


Keywords

BACTEC MGIT 960,Drug Resistance,Mycobacterium Tuberculosis, Lowenstein-Jensen medium, PCR
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  • Clinical Evaluation of Automated BACTEC MGIT 960 System for Identification, Recovery and Drug Susceptibility Testing of Mycobacterium tuberculosis Clinical Isolates

Abstract Views: 161  |  PDF Views: 0

Authors

Thirumurugan Ravibalan
Department of Microbiology, Intermediate Reference Laboratory, State TB Training and Demonstration Centre, Government Hospital for Chest Diseases, Gorimedu, Puducherry - 605006, India
Antony V Samrot
Department of Biotechnology, Sathyabama University, Rajiv Gandhi Road, Jeppiaar Nagar, Chennai - 600119, Tamilnadu, India
Kathirvel Maruthai
Department of Microbiology, Intermediate Reference Laboratory, State TB Training and Demonstration Centre, Government Hospital for Chest Diseases, Gorimedu, Puducherry - 605006, India
Vallayyachari Kommoju
Department of Microbiology, Intermediate Reference Laboratory, State TB Training and Demonstration Centre, Government Hospital for Chest Diseases, Gorimedu, Puducherry - 605006, India
Surendar Kesavan
Department of Microbiology, Intermediate Reference Laboratory, State TB Training and Demonstration Centre, Government Hospital for Chest Diseases, Gorimedu, Puducherry - 605006, India
Muthuraj Muthaiah
Department of Microbiology, Intermediate Reference Laboratory, State TB Training and Demonstration Centre, Government Hospital for Chest Diseases, Gorimedu, Puducherry - 605006, India

Abstract


Background and Objectives: The objective of this study was to evaluate the clinical applicability of BACTEC Mycobacterial Growth Indicator Tube 960TM system with Lowenstein-Jensen (LJ) culture for routine recovery and drug susceptibility test analysis of mycobacteria from clinical specimens. Method and Statistical Analysis: One hundred and seven clinical specimens were processed by routine laboratory procedures like p-nitro benzoic acid test and PCR amplification of IS6110 and inoculated in LJ medium and MGIT for mycobacterial growth recovery and DST procedures. Findings: Ninety four (87.8%) samples were demonstrated reproducible result by MGIT, in which 89 (83.1%) were smear positive and all the specimens were confirmed as M. tuberculosis complex by MGIT PNB and IS6110 PCR procedures. The observed mean time for mycobacterial detection was 9 days for primary culture and 11 days for DST in MGIT system. Applications/Improvements: The use of BACTEC MGIT 960 system was found to be a sensitive, rapid mycobacterial recovery and culture system and offers precise identification and detection of drug resistance from clinical isolates at earliest.


Keywords


BACTEC MGIT 960,Drug Resistance,Mycobacterium Tuberculosis, Lowenstein-Jensen medium, PCR



DOI: https://doi.org/10.17485/ijst%2F2016%2Fv9i8%2F131045