Quantification of Thiocolchicoside in Rabbit Serum by High Performance Liquid Chromatographic Method: Application to Pharmacokinetic Study

Objective: A sensitive and précised bioanalytical method for quantification of Thiocolchicoside (THC) in rabbit serum was developed using high performance liquid chromatographic technique. Aceclofenac (ACE) was used as Internal Standard (IS). Methods: Phenomenix Luna® C18 column (150 x 4.6mm.i.d.5 μ) was used for method development of THC.Ammonium di hydrogen phosphate buffer: Acetonitrile (60:40) was used as mobile phase where in pH was adjusted to 4. 5 with glacial acetic acid. Findings: Flow rate was set to 1.0 mL/min and elements were monitored at 264 nm by UV detector. The retention times detected were 3.16 and 4.25 minutes for THC & ACE respectively. Linearity of the calibration curve was in the range of 10- 4000 ng mL-1. Inter day, intra-day coefficient of variation and percent error values of the developed assay method was with the acceptable limits of less than 15 %. The mean recovery was recorded to be more than 98.6 and 99.5 % for THC and ACE respectively. Application: Validated bio-analytical method was successfully applied for estimation of THC in Rabbit serum after application of Transdermal films to the rabbit skin.