Liquid Chromatographic Enantioseparation of (RS) - Baclofen by Indirect Methods - A Review

Objective: This article surveys high-performance liquid chromatographic methods of Enantioseparation of (RS)- Baclofenstereiosomers. Methods: The methods adopted for enantioseparation of Baclofen was derivatizing Baclofen with a suitable Chiral Derivatizing Reagent (CDR) and then the resulting diasteroemrs were subjected on C 18 column. The diastereomers get separated due to different physical and chemical properties of diastereomers. Findings: Among several CDRs tested for enantiosepration of (RS) baclofen, Marfey’s reagent based CDR, FDNP-L-Leu-amide was found to have highest resolution of the Baclofen enantiomers. Applications: The chiral separation of pharmaceuticals is one of the major research topics in the pharmaceutical industry since it became evident that the potential biological or pharmacological applications are mostly restricted to one of the enantiomers, just like the (R)-enantiomer of Baclofen is about 100 times morepotent than the (S)-isomer, still it is sold as racemic drug. Therefore it was worthwhile to study the enantiosepration of (RS) Baclofen.