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Chemical Comparison by GC/MS Chromatography of Two Chemotypes of Essential Oils of Canaga odorata against Colletotrichum gloeosporioides


Affiliations
1 Departamento de Zootecnia, Universidad de Sucre, Colombia
 

Objectives: To determine the antagonistic activity of the essential oils of Cananga odorata flowers as a phytosanitary alternative against the phytopathogen Colletotrichum gloeosporioides. Methods/Statistical Analysis: The essential oils were obtained from C. odorata yellow flowers in the municipalities of Sincelejo and La Union, Sucre, by microwave-assisted hydrodistillation. The tests of antagonistic activity were carried out on the mycelial growth of C. gloeosporioides. The chemical profiles of the oils were made by GC/MS. With the results, the variance analysis and the Tukey test (p-value≤0.05) were performed for the significant differences. Findings: The results show that the flowers of C. odorata are a potential source of the raw material for the production of essential oils, due to their high yield, for which commercial exploitation is proposed. Presented the essential oil collected in Sincelejo the highest yields with 0.86% (v/w). In addition, the two essential oils presented antagonistic activity against C. gloeosporioides, being the one collected in Sincelejo the one that showed the highest averages of inhibition with 62.17%, followed by La Union with 57.38%. But at concentrations of 20,000 mg/L, 100% inhibition was found, similar to the positive control with benomyl. The MIC was 17,000 mg/L and 18,000 mg/L with 88.47% and 84.65% for Sincelejo and La Union, respectively. While the MFC was 100% at concentrations of 18,000 mg/L and 19,000 mg/L for Sincelejo and La Union, respectively. The analysis by GC/MS showed that the flowers of C. odorata collected in Sincelejo correspond to the Linalool chemotype and La Union al Bencil Benzoato. Improvements/Applications: Natural products represent a biotechnological alternative for phytosanitary problems, in this highly polluted planet, thanks to its high antagonistic capacity, zero environmental pollution and high biodegradability.
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  • Chemical Comparison by GC/MS Chromatography of Two Chemotypes of Essential Oils of Canaga odorata against Colletotrichum gloeosporioides

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Authors

Alexander Perez-Cordero
Departamento de Zootecnia, Universidad de Sucre, Colombia
Jaime de la Ossa-V
Departamento de Zootecnia, Universidad de Sucre, Colombia
Donicer Montes-Vergara
Departamento de Zootecnia, Universidad de Sucre, Colombia

Abstract


Objectives: To determine the antagonistic activity of the essential oils of Cananga odorata flowers as a phytosanitary alternative against the phytopathogen Colletotrichum gloeosporioides. Methods/Statistical Analysis: The essential oils were obtained from C. odorata yellow flowers in the municipalities of Sincelejo and La Union, Sucre, by microwave-assisted hydrodistillation. The tests of antagonistic activity were carried out on the mycelial growth of C. gloeosporioides. The chemical profiles of the oils were made by GC/MS. With the results, the variance analysis and the Tukey test (p-value≤0.05) were performed for the significant differences. Findings: The results show that the flowers of C. odorata are a potential source of the raw material for the production of essential oils, due to their high yield, for which commercial exploitation is proposed. Presented the essential oil collected in Sincelejo the highest yields with 0.86% (v/w). In addition, the two essential oils presented antagonistic activity against C. gloeosporioides, being the one collected in Sincelejo the one that showed the highest averages of inhibition with 62.17%, followed by La Union with 57.38%. But at concentrations of 20,000 mg/L, 100% inhibition was found, similar to the positive control with benomyl. The MIC was 17,000 mg/L and 18,000 mg/L with 88.47% and 84.65% for Sincelejo and La Union, respectively. While the MFC was 100% at concentrations of 18,000 mg/L and 19,000 mg/L for Sincelejo and La Union, respectively. The analysis by GC/MS showed that the flowers of C. odorata collected in Sincelejo correspond to the Linalool chemotype and La Union al Bencil Benzoato. Improvements/Applications: Natural products represent a biotechnological alternative for phytosanitary problems, in this highly polluted planet, thanks to its high antagonistic capacity, zero environmental pollution and high biodegradability.

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DOI: https://doi.org/10.17485/ijst%2F2018%2Fv11i39%2F130841