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The Usefulness of Comet Assay in Predicting Hematopoietic Disorders such as Myelodysplastic Syndrome and Acute Myeloid Leukemia


Affiliations
1 Department of Biology, Eritrea Institute of Technology, Mainefhi, Eritrea
2 Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, Japan, Japan
3 Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, Japan
 

The usefulness of 'comet assay' in predicting the hematopoietic disorders such as MDS and AML is reported. Bone marrow biopsies of de novo AML and MDS (3 from each) were subjected to alkaline comet assay along with suitable control. The results are based on the average tailmoment of about hundred randomly selected cells. Blood malignant samples showed significantly higher mean tailmoment (AML: 195±41.6 & MDS: 501±136.2) than the normal (29.66±6.1). In addition, the spontaneous occurrence of apoptotic cells was much higher in MDS (24.5% ±5); while in AML it was <1%. 'Comet assay' offers as single-step visual method to predict the hematopoietic neoplasia in suspected samples.

Keywords

Myelodysplastic Syndrome, Acute Myeloid Leukemia, Comet Assay, Apoptosis
User

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  • The Usefulness of Comet Assay in Predicting Hematopoietic Disorders such as Myelodysplastic Syndrome and Acute Myeloid Leukemia

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Authors

Natarajan Gajendran
Department of Biology, Eritrea Institute of Technology, Mainefhi, Eritrea
K. Tanaka
Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, Japan, Japan
T. S. Kumaravel
Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Hiroshima 734-8553, Japan

Abstract


The usefulness of 'comet assay' in predicting the hematopoietic disorders such as MDS and AML is reported. Bone marrow biopsies of de novo AML and MDS (3 from each) were subjected to alkaline comet assay along with suitable control. The results are based on the average tailmoment of about hundred randomly selected cells. Blood malignant samples showed significantly higher mean tailmoment (AML: 195±41.6 & MDS: 501±136.2) than the normal (29.66±6.1). In addition, the spontaneous occurrence of apoptotic cells was much higher in MDS (24.5% ±5); while in AML it was <1%. 'Comet assay' offers as single-step visual method to predict the hematopoietic neoplasia in suspected samples.

Keywords


Myelodysplastic Syndrome, Acute Myeloid Leukemia, Comet Assay, Apoptosis

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DOI: https://doi.org/10.17485/ijst%2F2007%2Fv1i1%2F29178