Understanding of MRHU-plasmids, Bacterial Adherence and Biomaterial in Clinical Application

Bacterial adherence has been recently reviewed by plasmid genetic properties of MRHU (D-mannose resistant haemagglutination of human erythrocytes) and change of surface antigens, fimbriae=pili, as an elongated protein appendages to facilitate transport of haemagglutine from cell to bacterial membrane, to lipopolysaccaride (LPS) and to hosts. The fimbriae=pili as expanding elongated protein appendages, have been recently characterised as BNT (Bionanotube) or as ionic channels. The transport of secondary metabolites, haemagglutinine passing through membrane to the external surface, may cause MRHU the colonizations. The mechanisms of MRHU (agglutinating RBC) and their pathogenic activities have been characterised against immune responses (IR) in Balb/C mice. Both the cells and fimbriae showed similar results at different time span. Based on observations, the author designed the concept of one 'Bio-material' to be used for continuous vaccinations and for slow IR, which will monitor the body infections, in case of chronic and acute bacterial diseases. The design reveals that if blood (serum) comes in contact with fimbriae=pili=BNT embeded immobilised matrix , it will cause IR, designed specifically against the infections of 08: 075: ETEC and 026:EPEC. MRHU (+) strains. These strains were essentially studied to isolate hybrid genetically engineered 5405 MRHU(+) auxotrophic E.coli K-12, non-pathogenic to Balb/c mice, produces at the same time antiadherent immune response to protect mice against fatal 026:EPEC diarrhea. The above results of HA- sero- typing and plasmid profiles supported the concept for the development of bio-materials, and matrix based slow vaccination process.