Indian Journal of Science and Technology

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Effect of NaCl on Biochemical Changes and Endophytic Fungal Assemblages in the Leaves of a Mangrove, Ceropsis roxiphorguna Arn


Affiliations
1 Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai – 600 025, India
2 Department of Botany, Annamalai University, Chidambaram – 608 002, India
 

One of the universal protective responses of plants to any type of stress is the accumulation of low-molecular organic substances such as amino acids, sugars and proteins. In this study, the effect of salinity on leaf pigments, proteins, free amino acids, proline, polyphenols, sugars and starch content was investigated. Status on endophytic fungal assemblages in the salt treated leaves of a mangrove, C. roxburghiana was studied under hydroponics pot culture. The photosynthetic pigments, sugars and protein concentrations of leaves were reduced by salinity and that the effect was aggravated by the long duration of salinity. Total contents of both chlorophyll and carotenoids decreased significantly by the treatment of NaCl. Total amino acid pool decreased by salinity, but the proline content increased only marginally. In the control, proline level did not change during the entire period of the study. The significant increase of accumulation of proline in leaves is implicated in osmotic adjustment to salinity. The endophytic fungal assemblage was also investigated. Among the 7 species, isolated Phyllosticta sp. was found frequently more when compared to other fungi. The results show that the endophytic fungi like Pestalotiopsis sp. and Phyllosicta sp. can be sustained and regenerated at low salinity condition in halophytes.

Keywords

Ceriops roxburghiana, Salinity, Biochemical Changes, Endophytic Fungi
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  • Alamgir ANM and Ali MY (1999) Effect of salinity on leaf pigments, sugar and protein concentrations and chloroplast ATPase activity of rice (Oryza sativa L.). Bangladesh J. Botany 28, 145–149.

  • Apse MP and Blumwald E (2002) Engineering salt tolerance in plants. Current Opinion in Biotechnology 13, 146-150.

  • Arnon DI (1949) Copper enzymes in isolated chloroplasts, polyphenol oxidase in Beta vulgaris. Plant Physiol.24, 1–15.

  • Bakes LS, Waldren RP and Teare LD (1973) Rapid determination of free proline for waterstress studies. Plant Soil. 39, 205-207.

  • Bills GF and Polishook JD (1991) Microfungi from Carpinus caroliniana. Can. J. Bot. 69,1477-1482.

  • Bills GF and Polishook JD (1992) Recovery of endophytic fungi from Chamaecyparis thyoides. Sydowia . 44,1-12.

  • Boyer JS (1982) Plant productivity and environment. Science. 218, 443-448.

  • Collado J, Platas G, Gonzales I and Pelaéz F (1999) Geographical and seasonal influence on the distribution of fungal endophytes in Quercus ilex. New Phytologist. 143, 525-532.

  • Delauney AJ and Verma DP (1993) Proline biosynthesis and osmoregulation in plants. The Plant Journal. 4, 215-223.

  • Dobranic JK, Johnson JA and Alikhan QR (1995) Isolation of endophytic fungi from eastern larch (Larix laricina) leaves from New Brunswick, Canada. Can. J. Microbiol. 41,194-198.

  • Dubey RS and Singh AK (1999) Salinity induces accumulation of soluble sugars and alters the activity of sugar metabolizing enzymes in rice plants. Biol. Plant. 42, 233-239.

  • Fisher PJ, Petrini O, Petrini LE and Sutton BC (1994) Fungal endophytes from the leaves and twigs of Quercus ilex L. from England, Majorea and Switzerland. New Phytol. 127,133-137.

  • Hata K and Futai K (1995) Endophytic fungi associated with healthy pine needles and needles infested by the pine needle gall midge Thecodiplosis japonensis. Can. J. Botany. 73,384-390.

  • Hernandez S, Deleu C and Larher F (2000) Proline accumulation by leaf tissues of tomato plants in response to salinity. Comptes Rendus de L Academie Des Sciences Serie III– Sciences de La Vie–Life Sciences. 323, 551– 557.

  • Heuer B (2003) Influence of exogenous application of proline and glycinebetaine on growth of salt-stressed tomato plants. Plant Sci. 165, 693-699.

  • Johnson JA and Whitney NJ (1989) An investigation of needle endophyte colonization patterns with respect to height and compass direction in a single crown of balsam fir (Abies balsamea). Can. J. Botany. 67,723-725.

  • Kerepesi I, Galiba G and Banyai E (1998) Osmotic and salt stresses induced differential alteration in water-soluble carbohydrate content in wheat seedlings. J. Agric. Food Chem. 46, 5347-5354.

  • Lee TM and Liu CH (1999) Correlation of decreases calcium contents with proline accumulation in the marine green macroalga Ulva fasciata exposed to elevated NaCI contents in seawater. J. Exp. Bot. 50, 1855– 1862.

  • Lowry OH, Rosebrough NJ, Farr AL and Randall RJ (1951) Protein measurement with the folin phenol reagent. J. Biol. Chem. 193, 265–275.

  • Makale P, Peltonen-Sainio P, Jokinen K, Pehu E, Setala H, Hinkkanen R and Somersalo S (1996) Uptake and translocation of foliarapplied glycinebetaine in crop plants. Plant Sci. 121, 221-230.

  • McCready RM, Guggolz J, Silviera V and Owens HS (1950) Determination of starch and amylase in vegetables. Anal. Chem. 22, 1156- 1158.

  • Moore S and Stein WH (1948) Photometric ninhydrin method for use in chromatography of amino acids. J. Biol.Chem. 176, 367–388.

  • Muthukumarasamy M, Gupta SD and Pannerselvam R (2000) Enhancement of peroxidase, polyphenol oxidase and superoxide dismutase activities by triadimefon in NaCl stressed Raphanus sativus L. Biol. Plant. 43, 317–320.

  • Nelson N (1944) A photometric adaptation of the Somogyi method for the determination of glucose. J. Biol.Chem. 153, 375–380.

  • Okane I, Nakagiri A and Ito T (1997) Endophytic fungi in leaves of ericaceous plant. Can. J. Botany 76, 657-663.

  • Reddy MP and Vora AB (1986) Changes in pigment composition, hill reaction activity and saccharides metabolism in bajra (Pennisetum typhoides S&H) leaves under NaCl salinity. Photosynthica, 20, 50-55.

  • Sahashi NY, Miyasawa T, Kubano S and Ito T (2000) Colonization of beech leaves by two endophytic fungi in northern Japan. Forest Pathology. 30,77-86.

  • Sieber TN (1989) Endophytic fungi in twigs of healthy and diseased Norway spruce and white fir. Mycological Res. 92, 322-326.

  • Somogyi M (1945) A new reagent for determination of sugars. J. Biol. Chem. 160, 61-68.

  • Tester M and Davenport R (2003) Na+ transport in higher plants. Ann. Bot. 91, 503-527.

  • Wang Y and Nil N (2000) Changes in chlorophyll, ribulose biphosphate carboxylaseoxygenase, glycine betaine content, photosynthesis and transpiration in Amaranthus tricolor leaves during salt stress. J. Hort. Sci. Biotech. 75, 623–627.

  • Yeo AR, Capron SJM and Flowers TJ (1985) The effect of salinity upon photosynthesis in rice (Oryza sativa L.): Gas exchange by individual leaves relation to their salt content. J. Exp. Bot. 36, 1240-1248.


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  • Effect of NaCl on Biochemical Changes and Endophytic Fungal Assemblages in the Leaves of a Mangrove, Ceropsis roxiphorguna Arn

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Authors

S. Kamalraj
Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai – 600 025, India
S. Sridevi
Department of Botany, Annamalai University, Chidambaram – 608 002, India
V. Gangadevi
Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai – 600 025, India
A. Venkatesan
Department of Botany, Annamalai University, Chidambaram – 608 002, India
J. Muthumary
Centre for Advanced Studies in Botany, University of Madras, Guindy Campus, Chennai – 600 025, India

Abstract


One of the universal protective responses of plants to any type of stress is the accumulation of low-molecular organic substances such as amino acids, sugars and proteins. In this study, the effect of salinity on leaf pigments, proteins, free amino acids, proline, polyphenols, sugars and starch content was investigated. Status on endophytic fungal assemblages in the salt treated leaves of a mangrove, C. roxburghiana was studied under hydroponics pot culture. The photosynthetic pigments, sugars and protein concentrations of leaves were reduced by salinity and that the effect was aggravated by the long duration of salinity. Total contents of both chlorophyll and carotenoids decreased significantly by the treatment of NaCl. Total amino acid pool decreased by salinity, but the proline content increased only marginally. In the control, proline level did not change during the entire period of the study. The significant increase of accumulation of proline in leaves is implicated in osmotic adjustment to salinity. The endophytic fungal assemblage was also investigated. Among the 7 species, isolated Phyllosticta sp. was found frequently more when compared to other fungi. The results show that the endophytic fungi like Pestalotiopsis sp. and Phyllosicta sp. can be sustained and regenerated at low salinity condition in halophytes.

Keywords


Ceriops roxburghiana, Salinity, Biochemical Changes, Endophytic Fungi

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DOI: https://doi.org/10.17485/ijst%2F2008%2Fv1i4%2F29233