Indian Journal of Science and Technology

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Sensor Based on Polyvinyl Chloride Immobilized Pseudomonas striata Cells as Metal-ionophore


Affiliations
1 Dept. of Biotech. & Environmental Sci, Thapar Univ., Patiala-147004, Punjab, India
2 School of Chemistry & Biochem., Thapar Univ., Patiala-147004, Punjab, India
 

PVC membrane containing lyophilized cell mass of Pseudomonas striata was prepared using dibutylpthalate as the plasticizer. Anionic interferences were excluded by use of potassium salt of tetrakis (4-chlorophenyl) borate (KTCIPB). The electrode was found to be fairly selective and sensitive for the zinc ions. It exhibited a linearity range of 10-1 to 10-5 M with near nernstian slope of 26.2 mV per decade. The membrane electrode showed a sharp response time of 6-10 sec and detection limit of 5x10-5 M at 25±1°C in the pH optima of 3-5. The interference was found to arise only from few transition metals such as Hg+2, Ag+ and Pb+2.

Keywords

Zn-metalloenzymes, Heavy Metal Determination, Potentiometric Biosensor, Ionophore
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  • Sensor Based on Polyvinyl Chloride Immobilized Pseudomonas striata Cells as Metal-ionophore

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Authors

Minakshi Datta
Dept. of Biotech. & Environmental Sci, Thapar Univ., Patiala-147004, Punjab, India
Susheel Mittal
School of Chemistry & Biochem., Thapar Univ., Patiala-147004, Punjab, India
Dinesh Goyal
Dept. of Biotech. & Environmental Sci, Thapar Univ., Patiala-147004, Punjab, India

Abstract


PVC membrane containing lyophilized cell mass of Pseudomonas striata was prepared using dibutylpthalate as the plasticizer. Anionic interferences were excluded by use of potassium salt of tetrakis (4-chlorophenyl) borate (KTCIPB). The electrode was found to be fairly selective and sensitive for the zinc ions. It exhibited a linearity range of 10-1 to 10-5 M with near nernstian slope of 26.2 mV per decade. The membrane electrode showed a sharp response time of 6-10 sec and detection limit of 5x10-5 M at 25±1°C in the pH optima of 3-5. The interference was found to arise only from few transition metals such as Hg+2, Ag+ and Pb+2.

Keywords


Zn-metalloenzymes, Heavy Metal Determination, Potentiometric Biosensor, Ionophore

References





DOI: https://doi.org/10.17485/ijst%2F2009%2Fv2i7%2F29496