Indian Journal of Science and Technology

Open Access Open Access  Restricted Access Subscription Access

Studies on Multidrug Resistant Pseudomonas Aeruginosa from Pediatric Population with Special Reference to Extended Spectrum Beta Lactamase


Affiliations
1 Department of Biotechnology, VELS University, Pallavaram, Chennai-600117, India
2 Department of Microbiology, Dr ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai -600 113, India
3 Department of Microbiology, SRM college of Arts and Science,Kattankulathur-603203, Tamilnadu, India
 

A total of 53 isolates of Pseudomonas aeruginosa were obtained from 250 clinical samples from pediatric populations in Chennai. The isolates were obtained from clinical specimens viz. the throat swab, ear swab and urine sample. The isolates of P. aeruginosa were subjected to antibiotic sensitivity test. Among various antibiotics tested, the strains showed highest resistance to ampicillin (85%), followed by amikacin (62.2%), norfloxacin (60.3%) and ciprofloxacin (50.9%). The data on ESBL production indicated overall production of 25% among MDR strains. MIC values for the antibiotics ranged from 3.9 μg/ml to 256µg/ml. These data suggest that the prevalence of ESBLs which are very important as these strains may often cause outbreaks in the pediatric population and causes increased morbidity and mortality in patients underlying diseases or limit therapeutic options due to the high degree of multidrug resistance.

Keywords

Multidrug Resistant, Pseudomonas Aeruginosa, ESBL, MIC, India
User

  • Agnihotri N, Gupta V and Joshi RM (2004) Aerobic bacterial isolates from burn wound infections and their antibiogram-a five year study. Burns. 30, 241-243.

  • Castanheira M, Toleman MA and Jones RN (2004) Molecular characterization of beta-lactamase gene, blagim-I, encoding a new subclass of metallo-betalactamase. Antimicrobial Agents Chemotherapy. 48, 4564-4661.

  • Colle JG, Miles RS and Wan B (1996) Tests for the identification of bacteria. In: Mackie and Mc Cartney Practical Medical Microbiology. Eds. Collee JG, Fraser AG, Marmion BP & Simmons A. 14th ed. Edinburg: Churchill Livingstone. pp: 131-50.

  • Fridkin SK and Gaynes RP (1999) Antimicrobial resistance in intensive care units. Clinical Chest Med. 20, 303-316.

  • Gladstone P, Rajendran P and Brahmadathan KN (2005) Incidence of carbapenem resistance nonfermenting gram negative bacilli from patients with respiratory infections in the intensive care units. Indian J. Med. Microbiol. 23 (3),189-191

  • Jarlier V, Nicolas MH and Fourneir G (1998) Extended spectrum β-lactamases conferring transferable resistance to newer β-lactam agents in Enterobacteriaceae: Hospital prevalence and susceptibility patterns. Rev. Infect. Dis.10, 867-878.

  • Mehta M, Punia JN and Joshi RM (2001) Antibiotic resistance in Pseudomonas aeruginosa strains isolated from various clinical specimen- a retrospective study. Indian J. Med. Microbiol. 19(4), 232.

  • Navaneeth BV, Sridaran D, Sahay D and Belwadi MR (2002) A preliminary study on metallo –beta-lactamase producing Pseudomonas aeruginosa in hospitalized patients. Indian J. Med. Res. 116, 264-267.

  • Niederman MS (2001) Impact of antibiotic resistance on clinical outcomes and the cost of care. Critical Care Med. 29, N114-N120.

  • Nordmann P and Naas T (1994) Sequence analysis of PER-1 extended spectrum beta-lactamase from Pseudomonas aeruginosa and comparison with class A beta lactamase. Antimicro Agents Chemotheraphy. 38,104-114.

  • Paladino JA, Sunderlin JL, Price CS and Schentag J (2002) Economic consequences of antimicrobial resistance. Surg. Infect. (Larchmont). 3, 259-267.

  • Poirel L, Weldhagen GF, De Champs C and Nordmann P (2002) A nosocomial outbreak of Pseudomonas aeruginosa isolates expressing the extended spectrum beta-lactamase GES-2 in South Africa. J. Antimicrobial.Chemotheraphy. 49, 561-565.

  • Poirel L, Le Thomas I, Naas T, Karim A and Nordmann P (2000) Biochemical sequence analysis of GES-1, a novel class A extended-spectrum betalactamase and the class 1 integron In52 from Klebsiella pneumonia. Antimicrob. Agents Chemotherapy. 44, 622-632.

  • Subha A and Ananthan S (2002) Extended spectrum β-lactamase mediated resistance to third generation cephalosporins among Klebsiella pneumoniae in Chennai. Indian J. Med. Microbiol. 20, 92-95.

  • Veenu Gupta, Rama Sehgal and Arora DR (2002) Mic values of antimicrobial agents against non-fermenting gram negative bacilli. 97 (12), 458-459.


Abstract Views: 404

PDF Views: 109




  • Studies on Multidrug Resistant Pseudomonas Aeruginosa from Pediatric Population with Special Reference to Extended Spectrum Beta Lactamase

Abstract Views: 404  |  PDF Views: 109

Authors

R. Amutha
Department of Biotechnology, VELS University, Pallavaram, Chennai-600117, India
Padmakrishnan
Department of Microbiology, Dr ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani Campus, Chennai -600 113, India
T. Murugan
Department of Microbiology, SRM college of Arts and Science,Kattankulathur-603203, Tamilnadu, India
M. P. Renuga Devi
Department of Microbiology, SRM college of Arts and Science,Kattankulathur-603203, Tamilnadu, India

Abstract


A total of 53 isolates of Pseudomonas aeruginosa were obtained from 250 clinical samples from pediatric populations in Chennai. The isolates were obtained from clinical specimens viz. the throat swab, ear swab and urine sample. The isolates of P. aeruginosa were subjected to antibiotic sensitivity test. Among various antibiotics tested, the strains showed highest resistance to ampicillin (85%), followed by amikacin (62.2%), norfloxacin (60.3%) and ciprofloxacin (50.9%). The data on ESBL production indicated overall production of 25% among MDR strains. MIC values for the antibiotics ranged from 3.9 μg/ml to 256µg/ml. These data suggest that the prevalence of ESBLs which are very important as these strains may often cause outbreaks in the pediatric population and causes increased morbidity and mortality in patients underlying diseases or limit therapeutic options due to the high degree of multidrug resistance.

Keywords


Multidrug Resistant, Pseudomonas Aeruginosa, ESBL, MIC, India

References





DOI: https://doi.org/10.17485/ijst%2F2009%2Fv2i11%2F29527