Indian Journal of Science and Technology

Open Access Open Access  Restricted Access Subscription Access

High Fructose Diet-induced Glucose Intolerance and Dyslipidemia in Adult Syrian Golden Hamsters


Affiliations
1 Division of Biochemistry, Central Drug Research Institute, Chhattar Manjil Palace, M.G. Road, Lucknow-226001, India
 

It has been hypothesized that glucose intolerance or diabetes can be induced in rodents by a high fructose diet (HFD: fructose 60%). This study was designed to examine the effects of HFD on various parameters viz fasting plasma glucose, glucose tolerance test, plasma triglycerides, cholesterol, HDL-C, LDL-C, NEFA (non-esterified fatty acid), glycerol, body weight, food consumption and water intake in male Syrian golden hamsters and the results were compared with a normal or standard diet (ND). Assessment after 3 months of dietary treatment reveals that the body weight gain, plasma triglycerides level, fasting plasma glucose and water intake were found significantly elevated (p < 0.05) in HFD group. Total Cholesterol, LDLcholesterol, NEFA and glycerol levels were also significantly elevated (p < 0.05) with concomitant level of decrease in HDL-C as compared to that of ND group. An oral glucose tolerance test showed a significant increase in plasma glucose levels after 2 h of glucose challenge in hamsters. During the experiment, it was noticed that important weight gain observed in HFD dietary test group was associated with a significant low (p < 0.001) food consumption. It is presumed that dietary nutrients contained in this high fructose diet might have an effect on insulin action and therefore, might contribute to the development of glucose intolerance and dyslipidemia. In addition to their diabetogenic effect, this HFD might probably have an atherogenic effect and can be used in a longterm study to induce diabetic dyslipidemia.

Keywords

Atherogenic, Diabetic Animal Model, Dyslipidemia, High Fructose Diet, Hypertriglyceridemia, Syrian Golden Hamster
User

  • Barnard, RJ, Roberts CK, Varon SM and Berger JJ (1998) Diet induced insulin resistance precedes other aspects of the metabolic syndrome. J. Appl. Physiol. 84 (4), 1311–1315.

  • Bonanome A and Grundy SM (1988) Effect of dietary Stearic acid on plasma cholesterol and lipoprotein levels. N. Engl. J. Med. 318, 1244– 1248.

  • Kamgang, R.Y. Mboumi, G.P. N'Dille and J.N. Yonkeu, (2005) Cameroon local diet-induced glucose intolerance and dyslipidemia in adult Wistar rat. Diabetes Res. Clin. Practice. 69 (3), 224–230.

  • Djite´ C (1995) Plan d’action Nationale sur la nutrition. Donne´es sur la zone e´cologique 4, Provinces de l’Ouest et Nord-Ouest, pp: 21–22.

  • Friedewald WT, Levy RI and Frederickson DS (1972) Estimation of the concenthamsterion of low density lipoprotein cholesterol in plasma, without use of prepahamsterive ultracentrifuge. Clin. Chem. 18, 499–502.

  • Ganong WF (1999) Review of Medical Physiology, 19th ed. Appleton and Lange, Stanford, pp: 267–269, 318– 339.

  • Grimaldi A, Cornet P, Masseboeuf N, Popelier M and Sachon C (1998) Guide Phamsterique du diabe`te, Mimi Editions. pp: 8–80, 162- 288.

  • Kim H, Aubert RE Herman WH (1998) Global burden of diabetes 1995–2025. Diabetes Care. 21, (9) 1414–1431.

  • Lindsey Benattar J, Pronchuck A and Hayes KC (1990) Dietary palmitic acid (16:0) enhances high density lipoprotein cholesterol and low density lipoprotein receptor mRNA in hamster (43145), PSEBM. 195, 261–269.

  • Matthews DR, Hosker JP, Rudenski AS, Naylor BA, Treacher DF and Turner RC (1985) Homeostasis model assessment: insulin resistance and beta-cell function from fasting plasma glucose and insulin concentrations in man. Diabetologia. 28, 412–419.

  • Ngogang J, Mbanya JC, Ngo Niobe S, Dongmo C and Carteret P (1996) Effects of palm oil and cotton seed oil on plasma cholesterol and triglycerides concenthamsterion in hamsters, FASBMB. p.92.

  • Noubissi Kamdem F (1998) Etudes des effets de Brillantaisia vogeliana (Nees) Benth. (Acanthace´es) sur le taux de lipides se´riques chez les hamsters, The`se de doctohamster, 3e`me cycle Biochimie Universite´ de Yaounde´ I, pp: 200.

  • Pagliassotti MJ, Prach PA, Koppenhafer TA and Pan DA (1996) Changes in insulin action, triglycerids and lipid composition during sucrose feeding hamsters. Am. J. Physiol. Regul. Integhamster. Comp. Physiol. 271, pp: R1316–R1326.

  • Pagliassotti MJEC, Gales DA, Podolin Yurin WCL and Morin. (2000) Developmental stage modifies diet-induced peripheral insulin resistance in hamsters, Am. J. Physiol. Regul. Integhamster. Comp. Physiol. 278, pp: R66– R73.

  • Pereira AT, Sinnaiah R and Das NP (1990) Effects of dietary palm oil on lipoprotein lipases: lipoprotein levels and tissue lipids in hamster. Biochem. Med. Metab. Biol. 44, 207– 217.

  • Portha B (1990) Les mode`les animaux de diabe`te non-insulinode´-pendant, in: G. Tchobroutsky, G. Slama, R. Assan, P. Feychet (Eds.), Traite´ de diabe´tologie, Pradel, Paris. pp: 317– 325.

  • Ranjana S, Fisch G, Teague B, Tamborlane VW, Banyas B, Allen K, et al. (2002) Prevalence of impaired glucose tolerance among, children and adolescents with marked obesity, N. Engl. J. Med. 346 (11), 802–810.

  • Reaven GM, Risser TR, Chen YDI and Reaven EP (1979) Characterization of a model of dietary-induced hypertriglyceridemia in young, non-obese hamsters. J. Lipid Res. 20, 371– 376.

  • Taghibiglou C, Carpentier A, Van Iderstine SC, Chen B, Rudy D, Aiton A, Lewis GF, and Adeli K (2000) Mechanisms of hepatic very low density lipoprotein overproduction in insulin resistance. Evidence for enhanced lipoprotein assembly, reduced intracellular ApoB degradation, and increased microsomal triglyceride transfer protein in a fructose-fed hamster model. J. Biol. Chem. 275, 8416–8425.

  • Thorburn AW, Storlien LH, Jenkins AB, Khouri S and Kraegen EW (1989) Fructose-induced in vivo insulin resistance and elevated plasma triglycerids levels in hamsters. Am. J. Clin. Vest. 49, 1155–1163.

  • Wakabayashi I and Kobaba (2002) WR Effet de l’aˆge sur le rapport entre le boire et les facteurs de risque athe´roscle´rotique. Gerontology. 48, 151–156.

  • Zavaroni I, Sander S, Scott S and Reaven GM (1980) Effects of fructose feeding on insulin secretion and insulin action in the hamster. Metabolism. 29 (10), 970–973.


Abstract Views: 468

PDF Views: 67




  • High Fructose Diet-induced Glucose Intolerance and Dyslipidemia in Adult Syrian Golden Hamsters

Abstract Views: 468  |  PDF Views: 67

Authors

Santosh Kumar Maurya
Division of Biochemistry, Central Drug Research Institute, Chhattar Manjil Palace, M.G. Road, Lucknow-226001, India
Arvind Kumar Srivastava
Division of Biochemistry, Central Drug Research Institute, Chhattar Manjil Palace, M.G. Road, Lucknow-226001, India

Abstract


It has been hypothesized that glucose intolerance or diabetes can be induced in rodents by a high fructose diet (HFD: fructose 60%). This study was designed to examine the effects of HFD on various parameters viz fasting plasma glucose, glucose tolerance test, plasma triglycerides, cholesterol, HDL-C, LDL-C, NEFA (non-esterified fatty acid), glycerol, body weight, food consumption and water intake in male Syrian golden hamsters and the results were compared with a normal or standard diet (ND). Assessment after 3 months of dietary treatment reveals that the body weight gain, plasma triglycerides level, fasting plasma glucose and water intake were found significantly elevated (p < 0.05) in HFD group. Total Cholesterol, LDLcholesterol, NEFA and glycerol levels were also significantly elevated (p < 0.05) with concomitant level of decrease in HDL-C as compared to that of ND group. An oral glucose tolerance test showed a significant increase in plasma glucose levels after 2 h of glucose challenge in hamsters. During the experiment, it was noticed that important weight gain observed in HFD dietary test group was associated with a significant low (p < 0.001) food consumption. It is presumed that dietary nutrients contained in this high fructose diet might have an effect on insulin action and therefore, might contribute to the development of glucose intolerance and dyslipidemia. In addition to their diabetogenic effect, this HFD might probably have an atherogenic effect and can be used in a longterm study to induce diabetic dyslipidemia.

Keywords


Atherogenic, Diabetic Animal Model, Dyslipidemia, High Fructose Diet, Hypertriglyceridemia, Syrian Golden Hamster

References





DOI: https://doi.org/10.17485/ijst%2F2008%2Fv1i6%2F29583