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Effect of Cyanobacterial Elicitor on Neem Cell Suspension Cultures


Affiliations
1 Dept. of Biosciences, Sri Sathya Sai University, Anantapur Campus, Anantapur-5150 001, A.P, India
2 P.G. Dept. of Botany, Queen Mary's College, Chennai 600004,T.N, India
 

The present study aimed to elucidate the effect of cyanobacterial elicitor derived from Anabaena sp. and Nostoc carneum on neem cell suspensions evolved from superior callus lines of Azadirachta indica A. Juss elite KN-1 accession with 12.88 mg/gm azadirachtin in its seed. Neem cell suspension cultures maintained in Murashige and Skoog liquid medium were treated with 265 cells/ml and 530 cells/ml homogenate of the elicitor on the third day. Growth of neem callus in terms of biomass revealed 5 fold enhancements when treated with 530 cells/ml elicitor on tenth day in callus line-1. In callus line-2 the log phase extended till tenth day recording a 6 fold increase with 265 cells/ml elicitor concentration. The type of callus line and concentration of the elicitor influence the biomass growth of neem callus in suspension culture. Enhanced azadirachtin production was achieved by algal elicitor treatment (as confirmed by HPLC analysis) and this study makes a step forward towards industrial manufacturing of the neem alkaloid.

Keywords

Anabaena sp., Nostoc Carneum, Neem, Azadirachtin, Elicitor, Suspension Culture
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  • Effect of Cyanobacterial Elicitor on Neem Cell Suspension Cultures

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Authors

B. Poornasri Devi
Dept. of Biosciences, Sri Sathya Sai University, Anantapur Campus, Anantapur-5150 001, A.P, India
A. Vimala
Dept. of Biosciences, Sri Sathya Sai University, Anantapur Campus, Anantapur-5150 001, A.P, India
Isha Sai
Dept. of Biosciences, Sri Sathya Sai University, Anantapur Campus, Anantapur-5150 001, A.P, India
S. Chandra
P.G. Dept. of Botany, Queen Mary's College, Chennai 600004,T.N, India

Abstract


The present study aimed to elucidate the effect of cyanobacterial elicitor derived from Anabaena sp. and Nostoc carneum on neem cell suspensions evolved from superior callus lines of Azadirachta indica A. Juss elite KN-1 accession with 12.88 mg/gm azadirachtin in its seed. Neem cell suspension cultures maintained in Murashige and Skoog liquid medium were treated with 265 cells/ml and 530 cells/ml homogenate of the elicitor on the third day. Growth of neem callus in terms of biomass revealed 5 fold enhancements when treated with 530 cells/ml elicitor on tenth day in callus line-1. In callus line-2 the log phase extended till tenth day recording a 6 fold increase with 265 cells/ml elicitor concentration. The type of callus line and concentration of the elicitor influence the biomass growth of neem callus in suspension culture. Enhanced azadirachtin production was achieved by algal elicitor treatment (as confirmed by HPLC analysis) and this study makes a step forward towards industrial manufacturing of the neem alkaloid.

Keywords


Anabaena sp., Nostoc Carneum, Neem, Azadirachtin, Elicitor, Suspension Culture

References





DOI: https://doi.org/10.17485/ijst%2F2008%2Fv1i7%2F29601