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Role of Insoluble Glycogen in Ethanol Adaptation Mechanism of Saccharomyces italicus


Affiliations
1 Dr. D. Y. Patil Biotechnology & Bioinformatics Institute, Survey No. 87/88, Mumbai–Pune Bypass Express Highway, Tathawade, Pune-411033, Maharashtra, India
2 Dr. D. Y. Patil Biotechnology & Bioinformatics Institute, Survey No. 87/88, Mumbai–Pune Bypass Express Highway, Tathawade, Pune-411033, Maharashtra
 

Saccharomyces italicus has two pools of glycogen-one is the soluble pool and the remainder water insoluble linked with cell wall or β-glucans. Soluble pool of glycogen present is assigned to produce energy required for metabolic activities but the insoluble pool in the cell wall plays a special physiological role in providing the osmotolerance under ethanol stress conditions. S. italicus grown in the presence of 2-8% v/v ethanol exhibits exponential increase in insoluble glycogen content as a protective measure against the stress created across the plasma membrane by the action of ethanol. But ethanol concentration above 8%v/v reduces hydrophobic interactions thereby decreasing the insoluble glycogen content with increase in membrane permeability.

Keywords

Saccharomyces, Glycogen, Osmotolerance, Ethanol, Yeast Cell Wall
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  • Role of Insoluble Glycogen in Ethanol Adaptation Mechanism of Saccharomyces italicus

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Authors

M. S. Dake
Dr. D. Y. Patil Biotechnology & Bioinformatics Institute, Survey No. 87/88, Mumbai–Pune Bypass Express Highway, Tathawade, Pune-411033, Maharashtra, India
M. B. Khetmalas
Dr. D. Y. Patil Biotechnology & Bioinformatics Institute, Survey No. 87/88, Mumbai–Pune Bypass Express Highway, Tathawade, Pune-411033, Maharashtra
S. V. Amarapurkar
Dr. D. Y. Patil Biotechnology & Bioinformatics Institute, Survey No. 87/88, Mumbai–Pune Bypass Express Highway, Tathawade, Pune-411033, Maharashtra

Abstract


Saccharomyces italicus has two pools of glycogen-one is the soluble pool and the remainder water insoluble linked with cell wall or β-glucans. Soluble pool of glycogen present is assigned to produce energy required for metabolic activities but the insoluble pool in the cell wall plays a special physiological role in providing the osmotolerance under ethanol stress conditions. S. italicus grown in the presence of 2-8% v/v ethanol exhibits exponential increase in insoluble glycogen content as a protective measure against the stress created across the plasma membrane by the action of ethanol. But ethanol concentration above 8%v/v reduces hydrophobic interactions thereby decreasing the insoluble glycogen content with increase in membrane permeability.

Keywords


Saccharomyces, Glycogen, Osmotolerance, Ethanol, Yeast Cell Wall

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DOI: https://doi.org/10.17485/ijst%2F2011%2Fv4i1%2F29932