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Optimization of Abiotic Conditions Suitable for the Production of Biodiesel from Chlorella vulgaris


Affiliations
1 Department of Plant Biotechnology, Presidency College, Chennai-600005, India
 

The present study reports the production of fatty acids by microalgae under the influence of light and dark condition. Microalgae are renewable resource containing rich lipids in their body and has the potential to refill the partial energy demands in an eco-friendly way. We isolated an indigenous green alga (Chlorella vulgaris) as a potent source for biodiesel. To get better yield of biofuel, the growth of the microalgal isolate was optimized with the addition of nutrients and salts under light and dark conditions. The lipid fractions were extracted from the biomass through solvent extractions and the fractions were analyzed for biodiesel under GC-MS. The percentage of lipids synthesized from C. vulgaris under light and dark conditions were analyzed and compared. The algae from dark sample shows rich in saturated fatty acid (capric acid, lauric acid&myristic acid) and considerable amount of PUFA (hexadecatrienoic acid, stearidonic acid, eicosapenaenoic acid, docosahexaenoic acid) when compare to algae grown under light. So the algae grown in dark condition is an excellent source for high yield of saturated fatty acids.

Keywords

Biofuel, Biodiesel, Chlorella vulgaris, Gasoline, Renewable Energy
User

  • Ackman RG, Tocher CS and McLachlan J (1968) Marine phytoplankter fatty acid. J. Fisheries Res. Bd. Can. 25, 1603–1620
  • Benemann JR, Goebel RP, Weissman JC and Augenstein DC (1982) Microalgae as a source of liquid fuels, final technical report to US dept. of energy. Washington DC, US dept. of energy. SAN-003-4-2.
  • Collyer DM and Fogg GE (1955) Studies on fat accumulation by algae. J. Exp. Bot. 6, 256-75.
  • Constantopolous G and Bloch K (1967) Effect of light intensity on the lipid composition of Euglena gracilis. J. Biol. Chem. 242, 3538–3542.
  • Fulton L (2004) Biomass and agriculture sustainability, markets and policies. International energy agency (IEA) biofuels study–interim report: Result and key messages so far. IEA, France, OECD Publication Service, pp105–112.
  • Greenspan P, Mayer EP and Fowler SD (1985) Nile red: A selective fluorescent stain for intracellular lipid droplets. J. Cell Biol. 100, 965–973.
  • Hu Q, Sommerfeld M, Jarvis E, Ghirardi M, Posewitz M, Seibert M and Darzins AI (2008) Microalgal triacylglycerols as feed stocks for biofuel production: Perspective and advances. Plant J. 54, 621-639.
  • Huang G, Chen F, Wei D, Zhang, X and Chen G (2010) Biodiesel production by microalgal biotechnology. Appl. Energy. 87, 38–46.
  • Khan SA, Rashmi Mir. Z. Husain, Prasad S and Banerjee UC (2009) Prospects of biodiesel production from microalgae in India. Renew. Sustain. Energy Rev. 13, 2361-2372.
  • Klyachko-Gurvich GL (1974) Changes in the content and composition of triacyl glyceride fattyacids during restoration of Chlorella pyrenoidosa cells after nitrogen starvation. Soviet Plant Physiol. 21, 611–618.
  • Matsunaga T, Matsumoto M, Maeda Y, Sugiyama H, Sato R and Tanaka T (2009) Characterization of marine microalga, Scenedesmus sp. strain JPCC GA0024 toward biofuel production. 31(9), 1367-1372.
  • Miao X and Wu Q (2006) Biodiesel production from heterotrophic microalgal oil. Biores. Technol. 97, 841–846.
  • Milne TA, Evans RJ and Nagle N (1990) Catalytic conversion of microalgae and vegetable oils to premium gasoline with shape selective zeolites. Biomass. 21, 219–232.
  • Pratoomyot J, Srivilas P and Noiraksar T (2005) Fatty acid composition of 10 microalgal species. J. Sci. Tech. 27(6), 1171-1189.
  • Rosen BH (1990) Identification manual For microalgae used in aquaculture. Florida Aqua Farms, Dade City, Florida. pp: 12-20.
  • Sharif Hossain ABM and Salleh A (2008) Biodiesel fuel production from algae as renewable energy. Amer. J. Biochem. Biotechnol. 4(3), 250-254.
  • Spoehr HA and Milner HW (1949) The chemical composition of Chlorella; effect of environmental conditions. Pl.Physiol. 24, 120-149.
  • Widjaja A (2009) Lipid production from microalgae as promising candidate for biodiesel production. Makara Teknologi. 13, (1), 47-51.
  • Yamada T and Sakaguchi K (1982) Electron microscopic studies of Chlorella ellipsoidea protoplast formation. J. Gen. Microbiol. 128(13), 19-1327.

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  • Optimization of Abiotic Conditions Suitable for the Production of Biodiesel from Chlorella vulgaris

Abstract Views: 494  |  PDF Views: 174

Authors

Sanniyasi Elumalai
Department of Plant Biotechnology, Presidency College, Chennai-600005, India
Velu Prakasam
Department of Plant Biotechnology, Presidency College, Chennai-600005, India
Ramganesh Selvarajan
Department of Plant Biotechnology, Presidency College, Chennai-600005, India

Abstract


The present study reports the production of fatty acids by microalgae under the influence of light and dark condition. Microalgae are renewable resource containing rich lipids in their body and has the potential to refill the partial energy demands in an eco-friendly way. We isolated an indigenous green alga (Chlorella vulgaris) as a potent source for biodiesel. To get better yield of biofuel, the growth of the microalgal isolate was optimized with the addition of nutrients and salts under light and dark conditions. The lipid fractions were extracted from the biomass through solvent extractions and the fractions were analyzed for biodiesel under GC-MS. The percentage of lipids synthesized from C. vulgaris under light and dark conditions were analyzed and compared. The algae from dark sample shows rich in saturated fatty acid (capric acid, lauric acid&myristic acid) and considerable amount of PUFA (hexadecatrienoic acid, stearidonic acid, eicosapenaenoic acid, docosahexaenoic acid) when compare to algae grown under light. So the algae grown in dark condition is an excellent source for high yield of saturated fatty acids.

Keywords


Biofuel, Biodiesel, Chlorella vulgaris, Gasoline, Renewable Energy

References





DOI: https://doi.org/10.17485/ijst%2F2011%2Fv4i2%2F29940