Indian Journal of Science and Technology

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Antibacterial Evaluation and Phytochemical Analysis of Medicago sativa L. against some Microbial Pathogens


Affiliations
1 Department of Microbiology, RVS College of Arts and Science, Sulur, Coimbatore–641 402, Tamil Nadu, India
 

Successive solvent extract viz., petroleum ether, chloroform, benzene, methanol, ethanol and water extracts of Medicago sativa was evaluated for antibacterial activity, against seven important bacterial strains by agar-well diffusion method. Methanol extract showed significant activity against all the tested bacteria followed by chloroform and ethanol extract. Benzene and Petroleum ether extracts did not show any significant activity. The antibacterial activity is more significant in solvent extracts compared to aqueous extract in all the plants indicating that the active principle responsible for antibacterial activity is more soluble in organic solvents. The results suggest that Medicago sativa is scientifically validate the use of this plant in the traditional medicine for isolation and characterization of the active principle for further exploitation in medical microbiology.

Keywords

Medicago sativa, Antibacterial Activity, Methanol, Well Diffusion, Phytochemical Analysis
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  • Antibacterial Evaluation and Phytochemical Analysis of Medicago sativa L. against some Microbial Pathogens

Abstract Views: 709  |  PDF Views: 259

Authors

A. Doss
Department of Microbiology, RVS College of Arts and Science, Sulur, Coimbatore–641 402, Tamil Nadu, India
V. Parivuguna
Department of Microbiology, RVS College of Arts and Science, Sulur, Coimbatore–641 402, Tamil Nadu, India
M. Vijayasanthi
Department of Microbiology, RVS College of Arts and Science, Sulur, Coimbatore–641 402, Tamil Nadu, India
Sruthi Surendran
Department of Microbiology, RVS College of Arts and Science, Sulur, Coimbatore–641 402, Tamil Nadu, India

Abstract


Successive solvent extract viz., petroleum ether, chloroform, benzene, methanol, ethanol and water extracts of Medicago sativa was evaluated for antibacterial activity, against seven important bacterial strains by agar-well diffusion method. Methanol extract showed significant activity against all the tested bacteria followed by chloroform and ethanol extract. Benzene and Petroleum ether extracts did not show any significant activity. The antibacterial activity is more significant in solvent extracts compared to aqueous extract in all the plants indicating that the active principle responsible for antibacterial activity is more soluble in organic solvents. The results suggest that Medicago sativa is scientifically validate the use of this plant in the traditional medicine for isolation and characterization of the active principle for further exploitation in medical microbiology.

Keywords


Medicago sativa, Antibacterial Activity, Methanol, Well Diffusion, Phytochemical Analysis

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DOI: https://doi.org/10.17485/ijst%2F2011%2Fv4i5%2F30060