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Characterization of a Natural Hemolysin in the Serum of a Hermit Crab Clibanarius longitarsus (Crustacea: Decapoda)


Affiliations
1 Department of Advanced Zoology & Biotechnology, Quaid-E-millat govt. college (W), Chennai-600 002, India
2 Department of Zoology, University of Madras, Guindy Campus, Chennai-600 025, India
 

A naturally occurring hemolysin (HL) was detected in the serum of the hermit crab Clibanarius longitarsus using mammalian RBC as indicator cells. The serum gave the highest HL titer with rabbit RBC. Cross adsorption of C. longitarsus serum with fixed rabbit RBC resulted in the complete removal of the lytic activity from the serum. The hemolytic activity in the serum of C. longitarsus was independent of divalent cations, reversibly sensitive to EDTA, thermolabile, stable between the pH 6-8. Further studies demonstrated that the HL activity is proteinaceous as it was precipitable by conventional deproteinizing agents and susceptible to the action of proteases and 2-mercapthoethanol. The HL-inhibition assay performed with several carbohydrates (mono, di, oligo, polysaccharides and Lipo polysaccharides) revealed that the serum HL was capable of specifically recognizing laminarin (a homopolymer of β 1, 3-glucan). Thus this hemolysin appears to be unique among all the known crustacean hemolysins.

Keywords

Hemolysin, Serum, Clibanarius longitarsus, Laminarin
User

  • Canicatti C (1987) Evolution of the lytic system in echinoderms. I. Naturally occurring hemolytic activity in Paracentrotus lividus (Echinoidea) coelomic fluids. Boll. Zool. 4, 325-329.

  • Canicatti C (1989) Evolution of the lytic system in echinoderms. II. Naturally occurring hemolytic activity in Marthasterias glacialis (Asteroidea) coelomic fluids. Comp. Biochem. Physiol. 93a, 587-591.

  • Canicatti C (1991) Binding properties of Paracentrotus lividus (Echinoidea) hemolysin. Comp. Biochem. Physiol. 98A, 463-468.

  • Canicatti C and Ciulla D (1988) Studies on Holothuria polii (Echinodermata) coelomocyte lysate. 11. isolation of coelomocyte hemolysins. Dev.Comp. Immunol. 12, 55-64.

  • Canicatti C and Parrinello N (1985) Hemagglutinin and hemolysin levels in the coelomic fluid from Holothuria polii (Echinodermata) following sheep erythrocyte. injection. Biol. Bull. 168, 175-1892.

  • Cooper EL, Rinkevich B, Uhlenbruck G and Valembois P (1992) Invertebrate immunity:Another view point. Scand.J.Immunol. 35, 247-266.

  • Decker JM, Elmholt A and Muchmore (1981) Spontaneous cytotoxicity mediated by invertebrate mononuclear cells toward normal and malignant vertebrate targets: inhibition by defined mono- and disaccharides. Cell. Immunol. 59, 161-170.

  • Dales RP (1982) Hemagglutinins and hemolysins in the body fluids of Neoamphitrite figulus and Arenicola marina (Annelida: polychaeta).Comp. Biochem. Physiol. 73A, 663-667.

  • Garvey JS, Cremer NE and Sussdorf DH (1979) Methods in immunology p.545, W.A. Benjamin Inc., Reading, Massachusetts.

  • Karp RD and Rheins LA (1980) Induction of specific humoral immunity to soluble proteins in the American cockroach Periplaneta Americana. 11. Nature of the secondary response. Dev.Comp.Immunol. 4, 629- 639.

  • Kisugi J, Kamiya H and Yamasaki M (1992) Biopolymers of marine invertebrates xii. A novel cytolytic factor from a hermit crab, Clibanarius longitarsus. Chem.Pharm.Bull. 40(6), 1641-1643.

  • Lehninger AL (1970) Biochemistry, Worth Publishers Inc., NY. pp: 833.

  • Leippe M and Renwrantz L (1988) Release of cytotoxic and agglutinating molecules by Mytilus hemocytes. Dev.Comp.Immunol.12, 297-308.

  • Lillie RD (1954) Histopathologic technique and practical histochemistry. The Blackiston Company Inc., NY. pp: 501.

  • Osada M, Ito T, Matsutani T and Mori K (1993) Partial purification and characterization of hemolysin from the coelomic fluid of Strongylocentrocentrotus nudus. Comp. Physiol. 105b, 43-49.

  • Parrinello N, Rindone D and Canicatti C (1979) Naturally occurring hemolytic system of the coelomic fluid of Holothuria polii Delle chiaie (Echinodermata). Dev. Comp. Immunol. 5, 33-42.

  • Parrinello N, Arizza V, Cammarata M and Parrinello DM (1993) Cytotoxic activity of Ciona intestinalis (Tunicata) hemocytes: Properties of the in vitro reaction against erythrocyte targets. Dev. Comp. Immunol. 17, 19-27.

  • Pearse AGE (1968) Histochemistry: Theoroetical and applied, Vol. I (3rd edn.), Churcill Livingston. pp: 759.

  • Ratcliffe NA, Rowley AF, Fitzgerald SW and Rhodes CP (1985) Invertebrate immunity: Basic concepts and recent advances. Int. Rev. Cytol. 97, 183-350.

  • Roch P (1979) Protein analysis of earthworm coelomic fluid :1 polymorphic system of the natural hemolysin of Eisenia fetida Andrei. Dev. Comp. Immunol. 3, 599-608.

  • Roch P, Valembois P, Davant N and Lassegues M (1981) Protein analysis of earthworm coelomic fluid- 11. Isolation and biochemical characterization of the Eisenia fetida Andrei factor (EFAF). Comp.Biochem.Physiol. 69B, 829-836.

  • Sindermann CJ (1971) Internal defence of crustacean: A review. Fish Bull. 69, 455-489.

  • Smith VJ and Chisholm JRS (1992) Non-cellular immunity in crustaceans. Fish & Shellfish Immunol. 2, 1-31.

  • Weinheimer PF, Evans EE, Stroud RM, Acton RT and Painter B (1969) Comparative immunology : Natural haemolytic system of the spiny lobster Panulinurus argus. Proc. Exp. Biol. Med. 130, 322-326.

  • Yoshino TP and Tai-Lan Tuan (1985) Soluble mediators of cytolytic activity in hemocytes of the Asian clam Corbicula fluminea. Dev.Comp.Immunol. 9, 515-522.


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  • Characterization of a Natural Hemolysin in the Serum of a Hermit Crab Clibanarius longitarsus (Crustacea: Decapoda)

Abstract Views: 419  |  PDF Views: 109

Authors

M. Shanmughavalli
Department of Advanced Zoology & Biotechnology, Quaid-E-millat govt. college (W), Chennai-600 002, India
M. Arumugam
Department of Zoology, University of Madras, Guindy Campus, Chennai-600 025, India

Abstract


A naturally occurring hemolysin (HL) was detected in the serum of the hermit crab Clibanarius longitarsus using mammalian RBC as indicator cells. The serum gave the highest HL titer with rabbit RBC. Cross adsorption of C. longitarsus serum with fixed rabbit RBC resulted in the complete removal of the lytic activity from the serum. The hemolytic activity in the serum of C. longitarsus was independent of divalent cations, reversibly sensitive to EDTA, thermolabile, stable between the pH 6-8. Further studies demonstrated that the HL activity is proteinaceous as it was precipitable by conventional deproteinizing agents and susceptible to the action of proteases and 2-mercapthoethanol. The HL-inhibition assay performed with several carbohydrates (mono, di, oligo, polysaccharides and Lipo polysaccharides) revealed that the serum HL was capable of specifically recognizing laminarin (a homopolymer of β 1, 3-glucan). Thus this hemolysin appears to be unique among all the known crustacean hemolysins.

Keywords


Hemolysin, Serum, Clibanarius longitarsus, Laminarin

References





DOI: https://doi.org/10.17485/ijst%2F2011%2Fv4i5%2F30066