![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextgreen.png)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextred.png)
Geographical Difference of Chromosome Aberrations between Japanese and American Small Cell Lung Cancer Cell Lines
Lung-cancer is the leading cause of cancer-related death worldwide. Cytogenetic analysis have been performed last two decades, but comparative analysis of karyotypes of small cell lung cancer (SCLC) from between Japan and America has not been precisely studied. Six Japanese and one American SCLC cell lines examined were hyperdiploid to neartetraploid with modal number of 52-91. These cells had a complex karyotype with more than 10 rearrangements. The karyotypic patterns were relatively consistent alterations involved long arm of chromosomes 1, 3, 7 and 11, and short arm of chromosomes 1 and 3. Higher rearrangements specially associated with translocations and deletions were observed in short and long arms of chromosomes 3 (3p21&3q25), and recurrently long arms of chromosome 7 (7q36 and 7q23). Although the chromosome of SCLC is too complex and G-banding analysis could not resolve all of many of the karyotypic abnormalities seen, several potentially site -specific abnormalities such as deletions of chromosome 3p, 7q and 3q, and amplifications of 3p, 7q, 1q, 11p, 2p and 12p in 6 SCLC cell lines established from chemotherapy resistant patients tumor cells. Losses of short arm chromosome 11 and 12 (11p, 12p) and long arm of chromosome 13 (13q), and amplifications of chromosomes 2, 11, 12, 13 and 19 (2p, 11p, 12p, 13q, 19p) were recurrently identified in the several cell lines, being different from published chromosomal abnormalities in American SCLC, which suggests geographical difference of SCLC karyotype. Also, these abnormal patters were largely different from non-small cell lung carcinoma (NSCLC). Unknown oncogenes localizing on these chromosome breakpoints for translocation or deletion region might be associated with the pathogenesis of SCLC. Present analysis can provide information on significant genes involved pathogenesis of SCLC.
Keywords
Chromosome Aberration, Lung Cancer
User
Information
- Ashman JN, Brigham J, Cowen ME, Greenman J, Lind M and Cawkwell L (2002) Chromosomal alterations in small cell lung cancer revealed by multicolour fluorescence in situ hybridization. Int. J. Cancer. 102(3), 230-236.
- Baik SH, Jee BK, Choi JS, Yoon HK, Lee KH, Kim YH and Lim Y (2009) DNA profiling by array comparative genomic hybridization (CGH) of peripheral blood mononuclear cells (PBMC) and tumor tissue cell in non-small cell lung cancer (NSCLC). Mol. Biol. Rep.36(7), 1767-1778.
- Bauch H, Johnson B, Hovis J, Yano T, Gazder A, Pettengill OS, Grasizano S, Sourenson GD, Poiesz BJ, Minna J, Linehan M and Zbar B (1987) Molecular analysis of the short arm of chromosome 3 in smallcell and non-small cell carcinoma of the lung. N. Engl.J. Med. 317, 1109-1113.
- Bello MJ, Moreno S and Rey JA (1989) Involvement of chromosomes 1, 3 and i(8q) in lung adenocarcinoma. Cancer Genet. Cytogenet. 38, 133- 135.
- Coe BP, Henderson LJ, Garnis C, Tsao MS, Minna J, Lam S, Macaulay C and Lam WL (2005) Highresolution chromosome arm 5p array CGH analysis of small cell lung carcinoma cell line. Genes Chrom. Cancer. 42(3), 308-331.
- Dehan E, Be-Dor A, Liao W, Lipson D, Frimer H, Rienstein S, Simansky D, Krupsky M, Yaron P, Friedman E, Rechavi G, Perlman M, Aviram-Goldring A, Izraeli S, Bittner M, Yakhini Z and Kaminski N (2007) Chromosomal aberrations and gene expression profiles in non-small cell lung cancer. Lung Cancer. 56, 175-184.
- Doyle AL, Giangiulo D, Hussain A, Park H-J, Chiu Yen R W and Borges M (1989) Differentiation of human variant small cell lung cancer cell lines to a classic morphology by retionoic acid. Cancer Res. 49, 6745-6751.
- Engelman JA, Zejnullahu K, Mitsudomi T, Song Y, Hyland C, Oh Park J, Linderman N, Gale Ch-M, Zhao X, Christensen J, Kosaka T, Holmes AJ, Rogers A, Cappuzzo F, Mok T, Lee Ch., Johson B, Cantley LC and Janne PA (2007) MET amplification leads to gefitinib resistance in lung cancer by activating ERBB3 signaling. Sci. 316, 1039-1043.
- Garnis C, Campbell J, Davies JJ, Macaulay C, Lam S and Lam WL (2005) Involvement of multiple developmental genes on chromosome 1p in lung tumorigenesis. Hum. Mol. Genet. 14(4), 475-482.
- Girard L, Zochbauer-Muller S, Virmani AK, Gazdar AF and Minna JD (2000) Genome-wide allelotyping of lung cancer identifies new regions of allelic loss, differences between small cell lung cancer and nonsmall cell lung cancer, and loci clustering. Cancer Res. 60(17), 4894-4906.
- Greulich H (2011) The genomics of lung adenocarcinoma: opportunities for targeted therapies. Genes & Cancer. 1(12), 1200-1210.
- Hartel PH, Shackelford AL, Hartel JV and Wenger SL (2008) Del (5) is associated with clinical and histological parameters in small cell neuroendocrine lung carcinoma. Int. J. Sug. Pathol. 16(4), 419-423.
- Heiway J and Betticher DC (2004) Lung : small cell cancer. Atras Genet Cytoget Oncol Haematol. June, 2004. URL:http//AtlasGeneticsOncology.org/Tumors?LungS mall CellIDI5142.html
- Inamura K and Ishikawa Y (2007) Microarry analysis for detection of metastasis and evaluation of prognosis in lung cancers. Jap. J. Lung Cancer. 48(4), 247:253.
- ISCN (2005) An International System for Human Cytogenetic Nomenclature. Shaffer LG & Tommerrup N (ed.), also in Cytogenetics & Genome Res. Karger, Basel.
- Jin YS, Mandahl N, Heim S, Schüller H and Mitelman F (1988) Isochromosomes i(8q) or i(9q) in three adenocarcinomas of the lung. Cancer Genet. Cytogenet. 33(1), 11-17.
- Kim YH, Kwei KA, Salari K, Kao J, Pacyna- Gengelbach M, Wang P, Hernandez-Boussard T, Gazder AF, Petersen I, Minna JD and Pollack JR (2010) Genomic and functional analysis identifies CRKL as an oncogene amplified in lung cancer. Oncogene. 29(10), 1421-1430.
- Kitada K, Yamasaki T and Aikawa S (2009) Amplification of the ABCB1 region accompanied by a short sequence of 200 bp from chromosome 2 in lung cancer cells. Cancer Genet. Cytogenet. 194(1), 4-11.
- Lee JS, Pathak S, Hopwood V, Tomosovic B, Mullins TD, Baker FL, Spitzer G and Neidhart JA (1987) Involvement of chromosome 7 in primary lung tumor and nonmalignant normal lung tissue. Cancer Res. 47, 6349-6352.
- Martin CA, Ziegler LM and Napoli JL (1990) Retinoic acud, dibutyl-cAMP, and differentiation affect the expression of retinoic acid receptors in F9 cells. Proc. Natl. Acad. Sci, USA. 87, 4804-4808.
- Meiju Ji, Haixia G, Cuixia G, Bingyn S and Peng H (2011) Highly frequent promotor methylation and PIK3CA amplification in non-small cell lung cancer (NSCLC). BMC Cancer 11.147, doi: 10.1186/1471:2407:11:147.
- McCaughan F, Pole JC, Bankier AT, Konforov BA, Falzon M, Rabbitts TH, George PJ, Dear PH and Rabitts PH (2010) Progressive 3q amplification consistently targets SOX2 in preinvasive squamous lung. Am. J. Respir. Crit. Care Med. 182(1), 83-91.
- Miller CT, Aggarwal S, Lin TK, Dagenais SL, Contreras JI, Orringer MB, Glover TW, Beer DG and Lin L (2003) Amplification and overexpression of the dual-specifity tyrosine-(Y)-phosphorylation regulated kinase 2(DYRK2) gene in esophageal and lung adednocarcinomas. Cancer Res. 63, 4136-4143.
- Miura I, Graziano SL, Cheng JQ, Doyle LA and Testa JR (1992) Chromosome alterations in human small cell lung cancer: frequent involvement of 5q. Cancer Res. 52, 1322-1328.
- Miura I, Siegfried JM, Resau J, Keller SM, Zhou J-Y and Testa JR (1990a) Chromosome alterations in 21 non-small cell lung carcinomas. Genes Chrom. Cancer. 2, 328-338.
- Miura I, Resau J, Tomiyasu T and Testa JR (1990b) Isochromosome (8q) in four patients with adenocarcinoma of the lung. Cancer Genet. Cytogenet. 48(2), 203-207.
- Mohr LC, Rodgers JK and Silvestri GA (2003) Gultathione S-transferase M1 polymorphism and the risk of lung cancer. Anticancer Res. 23(3A), 2111- 2124.
- Nagayama K, Kohno T, Sato M, Arai Y, Minna JD and Yokota J (2007) Homologous deletion scanning of the lung cancer genome at a 100-kb resolution, Genes Chrom. Cancer. 46, 1000-1010.
- Rygaard K, Sorenson GD, Pettengill OS, Cate CC, Spang-Thomsen M (1990) Abnormalities in structure and expression of the retinoblastoma gene in small cell lung cancer cell lines and xenografts in nude mice. Cancer Res. 50, 5312-4317.
- Salido M, Arriola E, Carracedo A, Cańnadas I, Rovira A, Espinet B, Rojo F, Arumi M, Serrano S, Albanell J and Sole F (2009) Cytogenetic characterization of NCI-H86 and NCI-H69AR small cell lung cancer cell lines by spectral karyotyping. Cancer Genet. Cytogenet. 191(2), 97-101.
- Sheilds PG (2002) Molecular epidemiology of smoking and lung cancer. Oncogene 21: 6870:6876.
- Testa JR, Siegfried JM, Liu Z, Hunt JD, Feder MM, Litwin S, Zhou JY, Taguchi T and Keller SM (1994) Cytogenetic analysis of 63 non-small cell lung carcinomas: recurrent chromosome alterations amid frequent and widespread genomic upheaval. Genes Chrom. Cancer. 11(3), 178-194.
- Ueda K, Cornwell MM, Gottesman M, Richer IB, Patan I,Roninson IB, Ling V and Riodan JR (1986) The mdr gene responsible for multidrug-resistance, codes for P-glycoprotein. Biochem. Biophy. Res. Commun. 141, 956-962.
- Nakagawa K, Yokota J, Wada M, Sasaki Y, Fujiwara Y, Sakai M, Muramatsu M, Terasaki T, Tsunokawa Y, Terada M et al., (1988) Levels of glutathione S transferase pi mRNA in human lung cancer cell lines correlate with the resistance to cicplastin and carboplatin. Jpn. Cancer Res. 79, 301-304.
- Wang-Peng J, Bunn Jr PA, Kao-Shan CS, Lee EC, Carney DN, Gazdar A and Minna JD (1982) A nonrandom chromosomal abnormality, del3p(14-23), in human small cell lung cancer(SCLC). Cancer Genet. Cytogenet. 6, 119-134.
- Wistuba II, Gazdere AF and Minna JD (2001) Molecular genetics of small cell lung carcinoma. Semi. Oncol. 2 Suppl. 4, 3-3.
- Yabuki N, Sakata K, Yamasaki T, Terashima H, Mio T, Miyazaki Y, Fujii T and Kitada K (2007) Gene amplification and expression in lung cancer cells with acquired paclitaxel resistance. Cancer Genet. Cytogenet. 173(1), 1-9.
- Yosimura K and Yamashita N (1982) Clinical statistical observation of 4,931 cases with lung cancer by histological types - Results of field study in Japan. Lung Cancer. 22(1), 1-16. (In Japanese)
- Yuan J, Knorr J., Altmannsberger M, Goeckenjan G, Ahr A., Scharl A and Strebhardt K (1999) Expression of p16 and lack of pRB in primary small cell lung cancer. J. Pathol. 189(3), 358-362.
- Zabarvsky ER, Lerman MI and Minna JD (2002) Tumor suppressor genes on chromosome 3p involved in the pathogenesis of lung and other cancers. Oncogene. 21(45), 6915-6935.
- Zech L, Bergh J and Nilsson K (1985) Karyotypic characterization of established cell lines and shortterm cultures of human lung cancers. Cancer Genet. Cytogenet. 15, 335-347.
![](https://i-scholar.in/public/site/images/abstractview.png)
Abstract Views: 522
![](https://i-scholar.in/public/site/images/pdfview.png)
PDF Views: 94