Indian Journal of Science and Technology

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Antimicrobial Protein Production by Bacillus amyloliquefaciens MBL27: Optimization of Culture Conditions Using Taguchi's Experimental Design


Affiliations
1 Microbiology Laboratory, Central Leather Research Institute (CSIR), Chennai 600 020, India
 

We report the applicability of the Taguchi DOE methodology for optimization of culture conditions for maximum antimicrobial protein (AMP) production by Bacillus amyloliquefaciens MBL27. The influence of individual factors and the relationships between the factors and their levels were established. Three factors viz, pH, incubation temperature and incubation period, each at three levels were selected and an orthogonal array (OA) layout of L27 containing 27 welldefined experiments were performed. Two response variables (bacterial growth and inhibitory activity of the AMP) were measured. Maximum AMP production of 6774.85 AU/ml against S. aureus was predicted at pH 7.0, incubation period 36 h and at temperature 30°C using response surface plots. This study serves as another example for the application of the Taguchi methodology for improvement of biological processes.

Keywords

Antimicrobial Protein, Antibiotic Production, B. amyloliquefaciens, Taguchi Design
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  • Antimicrobial Protein Production by Bacillus amyloliquefaciens MBL27: Optimization of Culture Conditions Using Taguchi's Experimental Design

Abstract Views: 570  |  PDF Views: 92

Authors

K. Vijayalakshmi
Microbiology Laboratory, Central Leather Research Institute (CSIR), Chennai 600 020, India
A. Premalatha
Microbiology Laboratory, Central Leather Research Institute (CSIR), Chennai 600 020, India
G. Suseela Rajakumar
Microbiology Laboratory, Central Leather Research Institute (CSIR), Chennai 600 020, India

Abstract


We report the applicability of the Taguchi DOE methodology for optimization of culture conditions for maximum antimicrobial protein (AMP) production by Bacillus amyloliquefaciens MBL27. The influence of individual factors and the relationships between the factors and their levels were established. Three factors viz, pH, incubation temperature and incubation period, each at three levels were selected and an orthogonal array (OA) layout of L27 containing 27 welldefined experiments were performed. Two response variables (bacterial growth and inhibitory activity of the AMP) were measured. Maximum AMP production of 6774.85 AU/ml against S. aureus was predicted at pH 7.0, incubation period 36 h and at temperature 30°C using response surface plots. This study serves as another example for the application of the Taguchi methodology for improvement of biological processes.

Keywords


Antimicrobial Protein, Antibiotic Production, B. amyloliquefaciens, Taguchi Design

References





DOI: https://doi.org/10.17485/ijst%2F2011%2Fv4i8%2F30898