Indian Journal of Science and Technology

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Investigation of Culture Filtrate of Endophytic Fungi Nigrospora Sp. Isolate Rs 10 in Different Concentrations towards Root-knot Nematode Meloidogyne Spp.


Affiliations
1 Department of Plant Protection, Faculty of Agriculture, Hasanuddin University, Indonesia
 

One of the modes of action of endophytic fungi against plant pathogens e.g. plant parasitic nematode, is the production of toxic culture filtrate. Experiments were conducted to investigate the antagonistic effects of different concentrations of the culture filtrate produced by the endophytic fungi Nigrospora sp. isolate RS 10 against the ischolar_main knot nematode <I>Meloidogyne</I> spp. <I>Nigrospora</I> sp. isolate RS 10 was isolated from the healthy ischolar_main of a sengon plant (<I>Paraserianthes falcataria</I>). Three different concentrations of the culture filtrate were investigated using in vitro bioassays against the motile juvenile stage 2 of the ischolar_main knot nematode Meloidogyne spp. Inactivation and mortality were recorded after 1, 3 and 24 hrs of exposure to culture filtrates. The mortality of the ischolar_main-knot nematode <I>Meloidogyne</I>-J2 after 24 hrs exposure to the culture filtrate was confirmed by rinsing with sterile distilled water that demonstrated the effects of the culture filtrate treatment were irreversible, as nematodes did not recover. Nematode mortality rates after 1 to 24 hr exposure periods ranged from 80 % to 100 %. The effects of the culture filtrates on ischolar_main-knot nematode <I>Meloidogyne</I>-J2 increased with increasing culture filtrate concentration.

Keywords

Endophytic Fungi, Culturfiltrate, Root-knot Nematode, Meloidogyne spp., Nigrospora sp.
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  • Becker J O, Mejia T E et al. (1988). Effects of rhizobacteria on ischolar_main-knot nematodes and gall formation, Phytopathology, vol 78, 1466–1469.

  • Cayrol J, and Djian, C. (1990). Etude de la toxicite de Fusarium roseum var arthrosporioides pour le nematode Meloidogyne arenaria, Comptes rendus de l’Académie d’agriculture de France, vol 76(8), 121–129.

  • Hallman J, and Sikora R A (1994). In vitro and in vivo control of Meloidogyne incognita with culture filtrates from non-pathogenic Fusarium oxysporum on tomato, Journal of Nematology, vol 26(1), 102.

  • Hayashi M, Wada K et al. (1981). New nematicidal metabolites from fungus, Irpex lactus, Agricultural Biology and Chemistry, vol 45(6), 1527–529.

  • Hooper D J, Hallmann J et al. (2005). Methods for extraction, processing and detection of plant and soil nematodes, Luc M, Sikora R A and Bridge J (Eds), Plant parasitic nematodes in subtropical and tropical Agriculture, CAB International, Wallingford, UK, 53–86.

  • Hussey R S, and Janssen G J W (2002). Plant resistance to parasitic nematodes, Root-knot nematodes: Meloidogyne species, Starr J L, Cook R, Bridge J (Eds.), CABI Publishing, 43–70.

  • Kiewnick S, and Sikora R A (2006). Biological control of the ischolar_main-knot nematode Meloidogyne incognita by Paecilomyces lilacinus strain 251, Biological Control 38, 179–187.

  • Larran S, Rollán C et al. (2002). Endophytic fungi in healthy soybean leaves, Investigación Agraria: Producción y Protección de Vegetales, vol 17, No. 1, 173–177.

  • Mani A, and Sethi C L (1984). Some characteristics of culture filtrates of Fusarium solani toxic to Meloidogyne incognita, Nematropica, vol 14(2), 121–129.

  • McInroy J A, and Kloepper J W (1995). Survey of indigenous bacterial endophytes from cotton and sweet corn, Plant Soil, vol 173, 337–342, DOI: 10.1007/BF00011472

  • Meyer S L F, Massoud S I et al. (2000). Evaluation of Trichoderma virens and Bulkholderia cepacia for antagonistic activity against ischolar_mainknot nematode, Meloidogyne incognita, Nematology, vol 2(8), 871–879.

  • Moens M, Perry R N et al. (2009). Meloidogyne species: a diverse group of novel and important plant parasites, Perry R N, Moens M, Starr J L (Eds.), Root-knot nemadodes, Wallingford, UK, CABI Publishing, 1–17.

  • Molina G C, and Davide R G (1986). Evaluation of microbial extracts for nematicidal activity against plant parasitic nematodes Meloidogyne incognita and Radopholus similes, The Phillipine Agriculturalist, vol 69, 173–186.

  • Amin N 1994. Untersuchungen über die Bedeutung endophytischer Pilze für die biologische Bekämpfung des wandernden Endoparasiten Radopholus similis (Cobb) Thorne an Bananen, PhD-Thesis, Bonn University.

  • Amin N, La Daha et al. (2008). The use of endophytic fungi to control of pest leaf borer Oryctes rhinoceros and Sexava sp. on oil palm plant, Research Report, Indonesian Ministry of Research and Technology.

  • Amin N, La Daha et al. (2011). Fungal endophyte as Biopesticide and Biofertilizer in Form of Tablet. Patent No. 00201100098.

  • Amin N (2012). Effect of ischolar_mainexudates of Sengon (Paraserianthes falcataria L. Nielsen) inoculated with the fungal Endophyte Nigrospora sp. on control of the ischolar_main-knot nematode Meloidogyne spp, Proceeding of International Conference on the Impacts of Climate Change to Forest Pests and Diseases in the Tropics, Jogyakarta, Indonesia.

  • Petrini O (1991). Fungal endophytes of tree leaves, Andrews J H, Hirano S S (Eds.), Microbial Ecology of Leaves, Springer-Verlag, NY, 179–187.

  • Racke J, and Sikora R A (1992). Isolation, formulation and antagonistic activity of rhizobacteria toward the potato cystnematode Globodera Pallida, Soil Biology & Biochemistry, vol 24(6), 521–526.

  • Sasser J N, and Freckman D W (1987). A world perspective on nematology: the role of the society, Veech J A, Dickson D W (Eds), Vistas on nematology: a commemoration of the twenty-fifth anniversary of the society of nematologists, 7–14.

  • Schuster R-P (1992). Untersuchungen zur biologischen Bekämpfung des Kartoffelzysten-nematoden Globodera pallida (Stone) mit eipathogenen Pilzen unter Berücksichtigung von deren Wirkungsweise sowie biotischen und abiotischen Faktoren. Diss, Bonn.

  • Siddiqui I A, and Shaukat S S (2003). Endophytic bacteria: prospects and opportunities for the biological control of plant–parasitic nematodes, Nematologia Mediterranea, vol 31, 111–120.

  • Sikora R A, Niere B et al. (2003). Endophytic microbial diversity and plant nematode management in African Agriculture, Neuenschwander P, Borgemeister C and Langewald J (Eds), Biological control in IPM systems in Africa, CAB International. Wallingford, 179–192.

  • Sikora RA, Schafer K et al. (2007). Modes of action associated with microbially induced in planta suppression of plant-parasitic nematodes, Australasian Plant Pathology, vol 36(2), 124–134.

  • Sturz A V, Christie B R et al. (1999). Endophytic bacterial communities in the periderm of potato tubers and their potential to improve resistance to soil-borne plant pathogens, Plant Pathology, vol 48, 360–369.

  • Ting A S Y , Meon S et al. (2008). Endophytic microorganisms as potential growth promoters of banana, BioControl, vol 53(3), 541–553. DOI: 10.1007/s10526-007-9093-1.

  • Ting A S Y, Meon S et al. (2007). Field evaluation of non-pathogenic Fusarium oxysporum isolates UPM31P1 and UPM39B3 for the control of Fusarium wilt in ‘Pisang Berangan’ (Musa, AAA), Proceeding of the International Symposium on Recent Advances in Banana Crop Protection for Sustainable Production and Improved Livelihoods, ISHS Acta Horticulturae, 139–144.

  • Wilson D (1995). Fungal endophytes which invade insect galls: insect pathogens, benign saprophytes, or fungal inquilines?, Oecologia, vol 103, 255–260.

  • van Loon L C, van Bakker P A H M, Pieterse C M J (1998). Systemic resistance induced by rhizosphere bacteria, Annual Review of Phytopathology, vol 36, 453–483.

  • Zareen A, Siddiqui I A et al. (2001). Observations on the nematicidal effect of Fusarium solani on the ischolar_main-knot nematode Meliodogyne javanica, Journal of Plant Pathology, vol 83, No. 3, 207–214.


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  • Investigation of Culture Filtrate of Endophytic Fungi Nigrospora Sp. Isolate Rs 10 in Different Concentrations towards Root-knot Nematode Meloidogyne Spp.

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Authors

Nur Amin
Department of Plant Protection, Faculty of Agriculture, Hasanuddin University, Indonesia

Abstract


One of the modes of action of endophytic fungi against plant pathogens e.g. plant parasitic nematode, is the production of toxic culture filtrate. Experiments were conducted to investigate the antagonistic effects of different concentrations of the culture filtrate produced by the endophytic fungi Nigrospora sp. isolate RS 10 against the ischolar_main knot nematode <I>Meloidogyne</I> spp. <I>Nigrospora</I> sp. isolate RS 10 was isolated from the healthy ischolar_main of a sengon plant (<I>Paraserianthes falcataria</I>). Three different concentrations of the culture filtrate were investigated using in vitro bioassays against the motile juvenile stage 2 of the ischolar_main knot nematode Meloidogyne spp. Inactivation and mortality were recorded after 1, 3 and 24 hrs of exposure to culture filtrates. The mortality of the ischolar_main-knot nematode <I>Meloidogyne</I>-J2 after 24 hrs exposure to the culture filtrate was confirmed by rinsing with sterile distilled water that demonstrated the effects of the culture filtrate treatment were irreversible, as nematodes did not recover. Nematode mortality rates after 1 to 24 hr exposure periods ranged from 80 % to 100 %. The effects of the culture filtrates on ischolar_main-knot nematode <I>Meloidogyne</I>-J2 increased with increasing culture filtrate concentration.

Keywords


Endophytic Fungi, Culturfiltrate, Root-knot Nematode, Meloidogyne spp., Nigrospora sp.

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DOI: https://doi.org/10.17485/ijst%2F2013%2Fv6i9%2F37130