![Open Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextgreen.png)
![Restricted Access](https://i-scholar.in/lib/pkp/templates/images/icons/fulltextred.png)
Molecular Characterization of Meghalaya Local Pigs (Niang Megha) using Microsatellite Markers
Molecular characterization using 21 microsatellite markers recommended by Food and Agricultural Organization of United Nations (FAO) for Swine revealed less genetic diversity in Meghalaya Local pig (Niang Megha). The loci used for investigation are informative. The number of observed alleles (Na) detected ranged from 2 to 6, with an overall mean of 3.9±1.30. A total of 82 alleles were observed across all the loci. The effective number of alleles (Ne) ranged from 1.145 to 4.2169 with a mean of 2.52±0.89. The frequency of allele values ranged from 0.0303 to 0.9333. The polymorphic information content (PIC) value ranged from 0.1227 to 0.7281. The overall means for observed (HO) and expected (He) heterozygosities were 0.545±0.279 and 0.548±0.179 respectively. The within-population inbreeding estimate (FIS) indicated heterozygosity deficiency of 0.0311. The Hardy-Weinberg equilibrium test revealed that 15 loci out of 21 deviated from equilibrium. The Shannon’s information index (I) was sufficiently high with a mean of 1 and bottleneck analysis revealed that population is not undergone any recent reduction.
Keywords
Meghalaya Local Pig (Niang Megha), Heterozygosity, Microsatellites, PIC
User
Information
- Sambrook J, Fristisch E F et al. (1989). Molecular Cloning: a Laboratory Manual, 2nd Edn., Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, USA.
- Henegariu O, Heerema N A et al. (1997). Multiplex PCR: critical parameters and step-by-step protocol, Biotechniques, vol 23(3), 504–511.
- Loffert D, Karger S et al. (1999). Optimization of multiplex PCR, Qiagen News, Issue 2, 5–8.
- Yeh F C, Boyle T et al. (1999). Popgene. Version 1.31. A Microsoft Windows based freeware for population genetic analysis, University of Alberta, Edmonton.
- Nei M (1978). Estimation of average heterozygosity and genetic distance from a small number of individuals, Genetics, vol 89, No. 3, 583–590.
- Cornuet J M, and Luikart G (1996). Description and power analysis of two tests for detecting recent population bottlenecks from allele frequency data, Genetics, vol 144, No. 4, 2001–2014.
- Food and Agriculture Organization (1998). In Guidelines for development of national farm animal genetic resources management plans, Measurement of Domestic Animal Diversity (MoDAD) recommended microsatellite markers, Food and Agriculture Organization of the United Nations, Rome, 19–24.
- Kaul R, Singh R K A et al. (2001). Evaluation of the genetic variability of 13 microsatellite markers in native Indian pigs, Journal of Genetics, vol 80(3), 149–153.
- Sollero B P, Paiva S R et al. (2010). Genetic diversity of Brazilian pig breeds evidenced by microsatellite markers, Livestock Science, vol 123(1), 8–15, DOI: 10.1016/j.livsci.2008.09.025.
- Swart H, Kortze A et al. (2010). Microsatellite-based characterization of Southern African domestic pigs (Sus scrofa domestica), South African Journal of Animal Science, vol 40(2), 121–132.
- Vicente A A, Carolino M I et al. (2008). Genetic diversity in native and commercial breeds of pigs in Portugal assessed by microsatellites, Journal of Animal Science, vol 86, No. 10, 2496–2507.
- Silva E P, and Russo C A M (2000). Techniques and statistical data analysis on molecular population genetics, Hydrobiologia, vol 420(1), 119–135.
![](https://i-scholar.in/public/site/images/abstractview.png)
Abstract Views: 487
![](https://i-scholar.in/public/site/images/pdfview.png)
PDF Views: 0