Journal of Biological Control

Open Access Open Access  Restricted Access Subscription Access

Standardization of Physiological Requirements, Substrates and Carrier Materials for Mass Production of Bacillus subtilis


Affiliations
1 Institute of Organic Farming, UAS Dharwad-580 005, Karnataka, India
 

Optimization of Bacillus subtilis growth conditions for mass production under laboratory conditions was investigated as part of a biological control programme. Aspects such as increasing yield using various culture media, pH, temperatures and carrier materials were studied. Nutrient broth (100×108) and 20 g molasses and 10 g yeast extract based media (98.60×108) gave the highest yield and 20 g molasses and 10g yeast extract broth was the most economical.  pH of 7-8, temperature of 30-350C and talc as carrier material were proved to best for mass production of B. subtilis.


Keywords

Bacillus subtilis, Carrier Material, Media, Temperature.
User
Notifications

  • Harwood CR. 1989, Introduction to the biotechnology of Bacillus. In: C.R. Harwood (Ed.). Biotechnology Handbooks, vol 2: Bacillus. 1 - 4. Plenum Press, London.

  • Korsten L., Cook N. 1996. Optimizing culturing conditions for Bacillus subtilis. South African Avacado Growers Association Yearbook 19: 54-58.

  • Nakkeeran S, Kavitha K, Chandrasekar G, Renukadevi P, Fernando WGD. 2006. Induction plant defense compounds by Pseudomonas chlororaphis PA23 and Bacillus subtilis BSCBE4 in controlling damping off of hot pepper caused by Pythium aphanidermatum. Biocontrol Sci Technol. 16 (4): 403- 416.

  • Norris JR, Berkeley RCW, Logan NA, Donnel AG. 1981. The genera Bacillus and Sporolactobacillus. p 71111742. In: M. Starr, H. Stolp, H.G. Truper, A. Balows and H. Starr M, Stolp H, Truper HG, Balows A, Schlegel H. (Eds) The Prokaryotes: A handbook on habitats, isolation, and identification of bacteria, vol 2: 1711 - 1742. Springer-Verlag, Berlin.

  • Peighmi-Ashnari S, Shariti-Tehrani A, Ahmadzadeh M, Behhoudi K. 2009. Interaction of different media on production and bioefficacy of Psuedomonas fluorescens P-35 and Bacillus subtilis B-3 against grey mold of apple. J Pl Pathol. 91 (1): 65-70.

  • Sharp J, Scawen MD, Atkinson T. 1989. Fermentation and downstream processing oí Bacillus. p 255-291 In: Harwood CR. (Ed.). Biotechnology handbooks, vol 2 Bacillus. Plenum Press, London.

  • Soleiman G, Masoud A. 2013. Optimization of a cost effective culture medium for the large scale production of Bacillus subtilis UTB96. Archives Phytopath Pl Prot 46: 1552-1563.


Abstract Views: 275

PDF Views: 128




  • Standardization of Physiological Requirements, Substrates and Carrier Materials for Mass Production of Bacillus subtilis

Abstract Views: 275  |  PDF Views: 128

Authors

H. Basamma
Institute of Organic Farming, UAS Dharwad-580 005, Karnataka, India
Shripad Kulkarni
Institute of Organic Farming, UAS Dharwad-580 005, Karnataka, India

Abstract


Optimization of Bacillus subtilis growth conditions for mass production under laboratory conditions was investigated as part of a biological control programme. Aspects such as increasing yield using various culture media, pH, temperatures and carrier materials were studied. Nutrient broth (100×108) and 20 g molasses and 10 g yeast extract based media (98.60×108) gave the highest yield and 20 g molasses and 10g yeast extract broth was the most economical.  pH of 7-8, temperature of 30-350C and talc as carrier material were proved to best for mass production of B. subtilis.


Keywords


Bacillus subtilis, Carrier Material, Media, Temperature.

References