Journal of Biological Control

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Optimization of Bacillus subtilis growth conditions for mass production under laboratory conditions was investigated as part of a biological control programme. Aspects such as increasing yield using various culture media, pH, temperatures and carrier materials were studied. Nutrient broth (100×108) and 20 g molasses and 10 g yeast extract based media (98.60×108) gave the highest yield and 20 g molasses and 10g yeast extract broth was the most economical.  pH of 7-8, temperature of 30-350C and talc as carrier material were proved to best for mass production of B. subtilis.


Keywords

Bacillus subtilis, Carrier Material, Media, Temperature.
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