Journal of Biological Control

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Surveys for entomopathogenic nematodes (EPNs) were carried out in eight localities covering three districts (Kangra, Sirmaur and Solan) of Himachal Pradesh, India. EPNs were recorded in two localities - Baijnath (Kangra) and Rajgarh (Sirmour), and were identified as Heterorhabditis bacteriophora. The potential of this nematode to kill the larvae of the cutworm, Agrotis segetum, was investigated at 10-40IJs / cm2 in Petri plates and in soil (at 1000-5000 IJs Kg-1) experiments. Studies on the comparative susceptibility of 3rd, 4th and 5th instar larvae of A. segetum indicated that the susceptibility of insect larvae to H. bacteriophora decreased with the increase in age. Insect mortality increased with the increase in the exposure time and inoculum level of the EPN. In the soil, 1000 IJs of H. bacteriophora Kg-1 were found to be sufficient to initiate the infection and kill up to 61.3 of 5th instar larvae of A. segetum after 7 days of exposure.

Keywords

Entomopathogenic Nematode, Heterorhabditis bacteriophora, Agrotis Segetum, Cutworm, Survey.
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