Amalgamation of Copper Nanoparticles of Assorted Size Using Nelumbo nucifera (Lotus) Leaf and its Bioelectrical Assay

There are several potential uses for green nanoparticle amalgamated in the medicinal and environmental sciences. Green synthesis specifically tries to reduce the use of harmful chemicals. For instance, it is often acceptable to employ organic resources like plants. In a single green synthesis step, biomolecules found in plant extract may transform metal ions into nanoparticles. This naturally occurring conversion of a metal ion to a base metal may be carried out quickly, conveniently, and at ambient temperature and pressure. In the current study, the production of CuNPs utilizing different-sized Nelumbo nucifera leaf extract has been reported. In order to determine how CuNPs generated, several techniques including UV-Visible, XRD, SEM, EDAX, FTIR, and cyclic voltammetry studies were used. The UV-Visible spectra of the amalgamated CuNPs show a peak between 250 and 450 nm. The morphology of CuNPs are spike in shapes with sizes of 33nm for 10mM and 25nm for 50mM, and the nanoparticles are crystalline in nature, according to the XRD and SEM examinations. The amalgamated CuNPs contain 37.55% copper, according to EDAX, and FTIR shows the absorption peak of copper at 1640 and 576 cm^-1. The oxidation and reduction of amalgamated CuNPs are visible by cyclic voltammetry. CuNPs have been put to the test against Staphylococcus aureus, Staphylococcus epidermidis, Klebsiella pneumoniae, Escherichia coli, and Pseudomonas aeruginosa for their antibacterial properties. CuNPs show the greatest zone of inhibition when used against Pseudomonas aeruginosa. Aspergillus flavus and Candida albicans have been used as test subjects for the antifungal testing of CuNPs. The CuNPs against Candida albicans show the largest zone of inhibition. CuNPs demonstrate strong antibacterial and antifungal efficacy, which means they have a considerable potential for application in the development of medications used to treat bacterial and fungal infections. The electrical potential difference of amalgamated CuNPs has been measured using a voltmeter and it is found that as concentration rises, so does the electrical potential difference.