Qualitative and Quantitative Estimation of Guggulsterone E and Z in Different Sodhit Guggul by LC-MS and HPLC Method

Objective: Guggul is the oleo-gum-resin obtained from deep incisions at the basal part of stem bark of Commiphora wightii belonging to Burseraceae family. It is very popular ancient Ayurvedic medicine used to cure various diseases. In Ayurveda guggul is always purified prior to use in different formulations. This process is known as sodhana. Guggulsterone E and Z are the prime constituents of Commiphora wightii. The main objective of this study was to identify the impact of guggul sodhana process mentioned in Ayurvedic Formulary of India (AFI) on the content of guggulsterone E and Z. The methanol extract of raw guggul and seven different sodhit guggul were qualitatively and quantitatively analysed by using LC-MS and HPLC methods. The LC-MS specrum indicated the presence of guggulsterone E&Z at m/z-313.2 in all samples with a retention time 4.8 min and 5.7 min respectively. The HPLC chromatogram at 245 nm showed guggulsterone E & Z with same retention time in all samples. The study reports that all the sodhit guggul samples contain guggulsterone E&Z. The water shodhit guggul showed 95.95% of total guggulsterone while triphala shodhit guggul showed 74.21%. It is suggested that sodhana process affects the quantity of guggulsterone E&Z but do not modify its chemical properties.