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In Vitro Antioxidant and Anticancer Activity of Plant Extracts of Tanacetum dolicophyllum (Kitam.) Kitam


Affiliations
1 School of Applied Sciences, Suresh Gyan Vihar University, Jaipur – 302017, Rajasthan, India
2 Synthetic Organic Chemistry Research Lab, Department of Chemistry, Thanthai Hans Roever College, Perambalur – 621212, Tamil Nadu, India
3 Faculty of Life Sciences, Mandsaur University, Mandsaur – 458001, Madhya Pradesh, India
 

The objective of this study was the assessment of antioxidant and cytotoxic activity from Tanacetum dolicophyllum. Soxhlet extraction has been used for preparing plant extracts for which five solvents have been used, i.e., Petroleum ether, benzene, ethyl acetate, ethanol, and water. Proteins, tannins, phlobatannins, amino acids, carbohydrates, alkaloids, steroids, flavonoids, glycosides, cardiac glycosides, and phenols were detected in the sample extracts. In the phytochemical screening of plant extracts, ethyl acetate and ethanolic extract show the best results which further leads to the investigation of antioxidant activity. Ferric-Reducing Antioxidant Power (FRAP), 2,2-diphenyl-2-picrylhydrazyl (DPPH), Reducing Power assay, and Phosphomolybdenum assays were performed to estimate antioxidant activity. In the RPA and TAC, the absorbance of sample extracts increases as there is an increase in concentration. In DPPH and FRAP assay, ethyl acetate showed good results. DPPH assay for ethyl acetate extract with 172.73(µg/ml) and ethanolic extract with 171.07(µg/ml) of IC50 values. The ethyl acetate extract had the highest antioxidant activity. The MTT assay was used to investigate the anticancer activity. The MTT assay discovered that the ethyl acetate extract had the highest anticancer activity against HeLa cells with an IC50 75 µg/mL as compared to a normal cell line J774A. Several compounds present in the extract of ethyl acetate acted as anticancer and contributed to cytotoxic activity.

Keywords

Alkaloids, Anticancer, Antioxidant, Cytotoxicity, Tanacetum dolicophyllum
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  • In Vitro Antioxidant and Anticancer Activity of Plant Extracts of Tanacetum dolicophyllum (Kitam.) Kitam

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Authors

Gunjan Sharma
School of Applied Sciences, Suresh Gyan Vihar University, Jaipur – 302017, Rajasthan, India
Zoya Zaidi
Synthetic Organic Chemistry Research Lab, Department of Chemistry, Thanthai Hans Roever College, Perambalur – 621212, Tamil Nadu, India
Mohammad Irfan Ali
Faculty of Life Sciences, Mandsaur University, Mandsaur – 458001, Madhya Pradesh, India
Sarmad Moin
School of Applied Sciences, Suresh Gyan Vihar University, Jaipur – 302017, Rajasthan, India

Abstract


The objective of this study was the assessment of antioxidant and cytotoxic activity from Tanacetum dolicophyllum. Soxhlet extraction has been used for preparing plant extracts for which five solvents have been used, i.e., Petroleum ether, benzene, ethyl acetate, ethanol, and water. Proteins, tannins, phlobatannins, amino acids, carbohydrates, alkaloids, steroids, flavonoids, glycosides, cardiac glycosides, and phenols were detected in the sample extracts. In the phytochemical screening of plant extracts, ethyl acetate and ethanolic extract show the best results which further leads to the investigation of antioxidant activity. Ferric-Reducing Antioxidant Power (FRAP), 2,2-diphenyl-2-picrylhydrazyl (DPPH), Reducing Power assay, and Phosphomolybdenum assays were performed to estimate antioxidant activity. In the RPA and TAC, the absorbance of sample extracts increases as there is an increase in concentration. In DPPH and FRAP assay, ethyl acetate showed good results. DPPH assay for ethyl acetate extract with 172.73(µg/ml) and ethanolic extract with 171.07(µg/ml) of IC50 values. The ethyl acetate extract had the highest antioxidant activity. The MTT assay was used to investigate the anticancer activity. The MTT assay discovered that the ethyl acetate extract had the highest anticancer activity against HeLa cells with an IC50 75 µg/mL as compared to a normal cell line J774A. Several compounds present in the extract of ethyl acetate acted as anticancer and contributed to cytotoxic activity.

Keywords


Alkaloids, Anticancer, Antioxidant, Cytotoxicity, Tanacetum dolicophyllum

References





DOI: https://doi.org/10.18311/jnr%2F2023%2F30460