Journal of Natural Remedies

K. Mukherjee , S. Badami , A. M. Wahile , S. Rajan , B. Suresh

Abstract

Objective: Evaluation of tyrosinase inhibitory activity of spices. Materials and methods: Ten different spices considered as usual commodity in Indian food habits was screened for their inhibitory activity against tyrosinase. The mushroom tyrosinase inhibitory activity was determined by dopachrome method using L-DOPA as the substrate. Results: Amongst the spices tested four spices viz. Turmeric (Curcuma longa ), cumin (Cuminum cyminum), black pepper (Piper nigrum), pipal (Ficus religiosa) showed the specific inhibitory activity against tryrosinase above 50% and out of them turmeric showed maximum inhibition which can be further explored for the characterization of the phytoconstituents. Other spices showed potential inhibition of tyrosinase activity. Conclusion: This finding could lead to the design and discovery of new tyrosinase inhibitors from Indian spices.

Keywords

Tyrosinase Inhibitor, Alcohol Extract, Piper Nigrum, Ficus Religiosa, Myristica Fragrance, Nigella Sativa, Cinnamomum Tamala, Murraya Koenigii, Coriandrum Sativum, Cuminum Cyminum, Carum Carvi, Curcuma Longa

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P. K. Mukherjee , K. Mukherjee , A. C. J. Hermans-lokkerbol , R. Verpoorte , B. Suresh

Abstract

Objective: Estimation of quercetin content of Eupatorium glandulosum and Coolebroke oppositifolia by HPLC. Materials and methods: The presence of quercetin (3, 3^I, 4^I, 5, 7 - pentahydroxyflavone) present in the leaves of Eupatorium glandulosum (Family- Asteraceae) and Coolebroke oppositifolia (Family : Labiatae) was quantitatively estimated by high performance liquid chromatography using reversed phase (RP-HPLC) with stepwise gradient elution on a RP C18 column. A two step elution with: acetonitrile- water- phosphoric acid (85%): from 16-83-1 to 32-67-1(v/v) was made. Results: The leaf extracts of E. glandulosum and C. oppositifolia contained 4.96 and 2.10 % and the respective powdered leaf contained 0.19 % (w/w) and 0.09 % (w/w) of quercetin . Conclusion: The method developed is useful to obtain quantitative values for quercetin present in these plant species, which may be useful for standardization of the same.

Keywords

Eupatorium Glandulosum, Coolebroke Oppositifolia, Flavonoid, Quercetin, RP-HPLC

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