Molecular Detection for Nosocomial Pseudomonas aeruginosa and its Relationship with multidrug Resistance, Isolated from Hospitals Environment

Pseudomonas aeruginosa is an important pathogen in hospitalized patient’s causative to their morbidity and mortality due to its multiple resistance mechanisms. Therefore, as a therapeutic option becomes restricted, the search for a new agent is a priority. So Pseudomonas aeruginosa is an extremely versatile Gram-negative bacterium capable of thriving in a broad spectrum of environments, and this performs main problems to workers in the field of doctors and nurses. One hundred and fifty samples were collected from different sources from Al-Kut hospitals, divided into two main groups: clinical (80)samples and (70) samples as a Nosocomial, collected from October to the December of the year 2018. All of these samples were cultured by specific and differential media, Forty (40) isolates of P.aeruginosa bacteria were identified by using microscopic examination, biochemical tests. The identification of (40) isolates of P.aeruginosa confirmed VITEK-2 system. The antibiotic sensitivity test recognized for all bacterial isolates and the results showed high sensitivity to amikacin, gentamicin, ciprofloxacin and Chloramphenicol, and high resistant to oxacillin and Cefoxitin. A molecular diagnosis is recognized by conventional PCR technique to detect the specified gene amplification products of the blaOXA-1 gene for Pseudomonas aeruginosa.