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Molecular characterization of Eae and Stxs genes for E. coli O157:H7 isolates from Calves


Affiliations
1 Department of Internal and Preventive Medicine, College of Vet. Med., University of Tikrit, Iraq
2 Department of Internal and Preventive Medicine, College of Vet. Med., University of Fallujah, Iraq
     

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This study aimed to detect eae and Stx genes of thirty two E. coli O157:H7 isolates recovered from calves. These isolates were isolated and identify by traditional methods of culturing and latex agglutination test from fecal swabs of four diarrheic calves and twenty eight apparently healthy calves. The eae and Stx genes were detected by a realtime PCR applied on the extracted bacterial DNA. The results showed that 19 isolates had stx1 gene, 10 isolates had stx2 gene and 17 isolates had eae gene. It is concluded that molecular characterization by using real time PCR is a good test for confirming infection with E. coli O157:H7 and detection of their eae and Stx genes

Keywords

Real time, PCR, stx, eae, Calves, E. coli O157:H7
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  • Molecular characterization of Eae and Stxs genes for E. coli O157:H7 isolates from Calves

Abstract Views: 441  |  PDF Views: 0

Authors

Jassim Mohamed Suleiman
Department of Internal and Preventive Medicine, College of Vet. Med., University of Tikrit, Iraq
Omar-Althani Shareef Saed
Department of Internal and Preventive Medicine, College of Vet. Med., University of Tikrit, Iraq
SMA AL-Kubaisi
Department of Internal and Preventive Medicine, College of Vet. Med., University of Fallujah, Iraq
Mustafa Salah Hasan
Department of Internal and Preventive Medicine, College of Vet. Med., University of Fallujah, Iraq
Maher Saber Owain
Department of Internal and Preventive Medicine, College of Vet. Med., University of Tikrit, Iraq
Mohammed Ali Hussein
Department of Internal and Preventive Medicine, College of Vet. Med., University of Fallujah, Iraq
Ahmed Sami Jarad
Department of Internal and Preventive Medicine, College of Vet. Med., University of Fallujah, Iraq

Abstract


This study aimed to detect eae and Stx genes of thirty two E. coli O157:H7 isolates recovered from calves. These isolates were isolated and identify by traditional methods of culturing and latex agglutination test from fecal swabs of four diarrheic calves and twenty eight apparently healthy calves. The eae and Stx genes were detected by a realtime PCR applied on the extracted bacterial DNA. The results showed that 19 isolates had stx1 gene, 10 isolates had stx2 gene and 17 isolates had eae gene. It is concluded that molecular characterization by using real time PCR is a good test for confirming infection with E. coli O157:H7 and detection of their eae and Stx genes

Keywords


Real time, PCR, stx, eae, Calves, E. coli O157:H7



DOI: https://doi.org/10.37506/v20%2Fi1%2F2020%2Fmlu%2F194421