Molecular Detection of Some Antibiotic Resistant Genes of Bacteria Isolated from Bloodstream Infections of Hemodialysis Patients

A cross-sectional hospital based study was done from November 2017 to February 2019 including 240 patients with chronic renal disease (168 males and 72 females) whose ages were between 15-75 years and they admitted to hemodialysis unit of Kirkuk general hospital. The study also included 50 individuals of blood donors as control group. Ten ml of blood were collected by vein puncture each patient and control enrolled in this study, blood were injected in blood culture bottle sterilely for isolation of bacteria causing bacteremia, with detection of CTX-M-G2 and Van A genes by real time PCR. The study showed that 32.25% of HD patients have positive blood culture while no one of blood donors have a positive blood culture. The highest rate of isolated bacteria from HD patients (51.28%) was G-ve bacteria comparing with G+ve bacteria, while 42.86% of isolated bacteria from patients with UTI was G+ve bacteria. although the result was non-significant relation. The highest rate of bacteria isolated from HD patients (25.65%) was S. aureus followed by E. faecalis (17.95%). The study showed that 80% of S. aureus isolates who were resistant to ceftriaxone were positive for CTX-M-G2 gene comparing with 20% haven’t CTX-M-G2 A gene. The highest rate of ceftriaxone resistant S. faecalis (77.78%) were positive for CTX-M-G2 gene comparing with 22.22% haven’t the gene, although the results was non-significant. The study showed that the highest rate of ceftriaxone resistant K. pneumoniae (63.64%) were positive for CTX-M-G2 gene comparing with 36.36% haven’t the gene. The study showed that 77.78% of S. aureus were positive for Van A gene comparing with 22.22% haven’t Van A gene. The highest rate of vancomycin resistant S. faecalis (69.23%) were positive for Van A gene comparing with 30.77% haven’t the gene. The study showed that all vancomycin resistant K. pneumoniae were negative for Van A gene. It was concluded that Most S. aureus and E. faecalis isolates were positive for CTX-M-G2 and vanA gene and most Klebsiella spp. isolates were harbored CTX-M-G2 gene