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Genotyping Diversity of Echerichia coli isolated from UTI in Iraqi Patients


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1 Middle Technical University, College of Health and Medical Technology, Baghdad, Iraq
     

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Background: Escherichia coli (E. coli) is the predominant uropathogen isolated in acute, communityacquired uncomplicated UTIs in adults and children. Patients and Methods: A total of (150) clinical samples (Urine) where collected from the patients at admission to the hospital in a sterile container. A loopful of uncentrifuged urine samples was cultured onto blood agar, MacConkey agar, HiCrome UTI Agar and EMB media,the diagnosis of E. coli bacteria was confirmed by using VITEK 2 system. Antimicrobial susceptibility testing of E. coli isolates was carried out against the antibiotics by Disc Diffusion Method, ERIC PCR used for determined genotyping diversity. Results: E. coli showed antibiotic resistant to Garamycine (86%), Ciproflaxcin (66%), Ceftriaxone (46%) and Rifampcine (100%). The biofilm formation result showed out of 50 E. coli isolate (12%) were weak biofilm formation, (72%) were moderate biofilm formation and (16%) were strong biofilm formation. Genotyping by ERIC-PCR (Enterobacterial Repetitive Intergenic Consensus) of 50 E. coli isolates subjected to ERIC-PCR, 47 isolates have amplications ranging from 700 to < 3000bp The generated banding profile showed highly polymorphic DNA fragments in these 47 isolates Conclusion: E. coli resistance to Garamycine (86%), Ciproflaxcin (66%), Ceftriaxone (46%) and Rifampcine (100%). The biofilm formation result 50 E. coli isolate (12%) were weak biofilm formation, (72%) were moderate biofilm formation. Genotyping by ERIC-PCR (Enterobacterial Repetitive Intergenic Consensus) of E.coli isolates subjected to ERIC-PCR, 47 isolates have amplications ranging from 700 to < 3000bp The generated banding profile was highly polymorphic DNA fragments in these isolates.

Keywords

E. coli, UTI , ERIC PCR, Biofilm, Genotype.
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  • Genotyping Diversity of Echerichia coli isolated from UTI in Iraqi Patients

Abstract Views: 388  |  PDF Views: 0

Authors

Tabarek Saeb Sabri
Middle Technical University, College of Health and Medical Technology, Baghdad, Iraq
Amal A. Kareem
Middle Technical University, College of Health and Medical Technology, Baghdad, Iraq

Abstract


Background: Escherichia coli (E. coli) is the predominant uropathogen isolated in acute, communityacquired uncomplicated UTIs in adults and children. Patients and Methods: A total of (150) clinical samples (Urine) where collected from the patients at admission to the hospital in a sterile container. A loopful of uncentrifuged urine samples was cultured onto blood agar, MacConkey agar, HiCrome UTI Agar and EMB media,the diagnosis of E. coli bacteria was confirmed by using VITEK 2 system. Antimicrobial susceptibility testing of E. coli isolates was carried out against the antibiotics by Disc Diffusion Method, ERIC PCR used for determined genotyping diversity. Results: E. coli showed antibiotic resistant to Garamycine (86%), Ciproflaxcin (66%), Ceftriaxone (46%) and Rifampcine (100%). The biofilm formation result showed out of 50 E. coli isolate (12%) were weak biofilm formation, (72%) were moderate biofilm formation and (16%) were strong biofilm formation. Genotyping by ERIC-PCR (Enterobacterial Repetitive Intergenic Consensus) of 50 E. coli isolates subjected to ERIC-PCR, 47 isolates have amplications ranging from 700 to < 3000bp The generated banding profile showed highly polymorphic DNA fragments in these 47 isolates Conclusion: E. coli resistance to Garamycine (86%), Ciproflaxcin (66%), Ceftriaxone (46%) and Rifampcine (100%). The biofilm formation result 50 E. coli isolate (12%) were weak biofilm formation, (72%) were moderate biofilm formation. Genotyping by ERIC-PCR (Enterobacterial Repetitive Intergenic Consensus) of E.coli isolates subjected to ERIC-PCR, 47 isolates have amplications ranging from 700 to < 3000bp The generated banding profile was highly polymorphic DNA fragments in these isolates.

Keywords


E. coli, UTI , ERIC PCR, Biofilm, Genotype.



DOI: https://doi.org/10.37506/v20%2Fi1%2F2020%2Fmlu%2F194502