Protective Immunity against Virulence Uropathogenic Escherichia Coli in Mice

Infection in the urine tract is more dominate as a medical condition around the world in the hospital and society, especially from young women and pregnant women. The reason for disease the first is uropathogenicEscherichia coli, which has several agents that help in the development that disease in addition to its the capability to stick on a host epithelial cell in urine tract with an assembly the biofilm, which is the most important factor for the disease. Therefore, a vaccine was made for these local isolates to avoid recurrent urinary tract infection and to obtain protective immunity by a laboratory mouse model. After isolation and diagnosis of 90 positive isolates using chrome agardiagnostic from women with urinary tract infection from hospitals in middle Iraq to Wasit province for the period from Jan. 2019 to Apr. 2019. All isolates were tested to detect virulence factors. One isolate was chosen for the determine LD₅₀, immunization and vaccine model, then to estimate the antibody (IgG) level during immunization in the serum of mice at 6 weeks. Results proved that isolates showed positive (71.11%) biofilm, (23.33%) α-heamolysin, (31.11%) serum resistance, and (41.11%) MRHA and (5.55%) MSHA , while LD₅₀=(1*10¹⁰).There are highly significant differences when using isolate in the level of IgG reaches the high peak ranged (749.1 ± 20.4) in 6 weeks as compared with control. The results showed that the local vaccine for this isolation provided preventive protection by inducing a protective immune response in mice to protect against infection.